杏仁种皮黑色素对果蝇的紫外辐射保护作用-河南农业科学.PDFVIP

※营养卫生 食品科学 2012, Vol.33, No.21 285 1 2 2, * 1,* 李红姣 ，张双凤 ， 李巨秀 ，赵 忠 (1.西北农林科技大学林学院，陕西 杨凌 712100 ；2.西北农林科技大学食品科学与工程学院，陕西 杨凌 712100) 摘 要：以果蝇西农12和果蝇中农e为实验模型，对果蝇进行紫外辐射处理后，在添加杏仁种皮黑色素的培养基中 进行培养，通过测定果蝇繁殖力、寿命、超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、蛋白质量浓度、总抗氧 化能力(T-AOC)等指标来研究杏仁种皮黑色素的抗紫外辐射活性。结果表明：紫外辐射组与对照组相比，两品系果 蝇的繁殖力、寿命、总抗氧化能力显著降低，蛋白质量浓度、超氧化物歧化酶活力呈现先增后降趋势，丙二醛含量 显著升高。当添加杏仁种皮黑色素质量浓度范围为0.02～0.2mg/mL时，与2min UV组相比，能显著提高两品系果蝇 的繁殖力，但所设添加黑色素的实验组中相间组的果蝇繁殖力没有达到显著水平；与5min UV 组相比，添加黑色素 能显著提高两品系果蝇的寿命、蛋白质量浓度、总抗氧化能力、SOD活力，降低MDA含量。杏仁种皮黑色素对果 蝇具有抗紫外辐射保护作用。 ：杏仁；黑色素；果蝇；紫外辐射 Anti-UV Irradiation Effect of Melanin Derived from Apricot Kernel Skin in Drosophila 1 2 2, * 1,* LI Hong-jiao ，ZHANG Shuang-feng ，LI Ju-xiu ，ZHAO Zhong (1. College of Forestry, Northwest AF University, Yangling 712100, China； 2. College of Food Science and Engineering, Northwest AF University, Yangling 712100, China) Abstract ：After UV irradiation treatment, two Drosophila strains, Xinong 12 and Drosophila Zhongnong e, were cultured in a medium supplemented with melanin derived from apricot kernel skin (AS-M). Fecundity, lifetime, SOD activity, MDA content, protein concentration, total antioxidant capacity (T-AOC) were measured to evaluate the anti-UV irradiation effect of AS-M. Compared with control group, UV irradiation substantially reduced fecundity, lifetime and T-AOC in both Drosophila strains, protein concentration and SOD activity tended to fi rst increase and then decrease, and MDA content markedly increased. AS-M addition at concentrations varying from 0.02 to 0.2 m