杜仲粕中桃叶珊瑚苷的分离和纯化-食品科学.PDFVIP

32 2011, Vol. 32, No. 02 食品科学 ※工艺技术 1, 2 1, 2 1 1 1 1, 2 李 辉 ，文赤夫 ，李亚男 ，彭玉丹 ，汪 兰 ，张永康 (1.吉首大学生物资源与环境科学学院，湖南 吉首 416000 ； 2.吉首大学 植物资源保护与利用省级重点实验室，湖南 吉首 416000) 为研究大孔树脂对桃叶珊瑚苷的吸附与解吸性能及分离纯化效果，通过静态吸附与解吸实验从 12 种大孔 树脂中筛选高性能树脂，用树脂进行柱层析分离目标产物并优化分离条件。结果表明：大孔树脂 SIPI-7 、HZ-820 和 S-8 对目标分子既有高的吸附率，又能有效脱附；当用 HZ-820 型树脂进行柱层析分离纯化桃叶珊瑚苷时，优化 分离条件为上样量0.5g/g(以生药质量:树脂质量计)、乙醇- 水- 冰醋酸混合溶剂(50:50:3，V/V)为洗脱剂、流速 1.0mL/min。 在优化条件下，收集含产物浓度高的流分，经浓缩和重结晶后所获产品中桃叶珊瑚苷纯度高达 96.82% ，且具有可 观的产品得率。 桃叶珊瑚苷；分离纯化；大孔树脂；杜仲粕 Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal 1,2 1,2 1 1 1 1,2 LI Hui ，WEN Chi-fu ，LI Ya-nan ，PENG Yu-dan ，WANG Lan ，ZHANG Yong-kang (1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China； 2. Hunan Provincial Key Laboratory of Plant Resource Protection and Exploitation, Jishou University, Jishou 416000, China) Abstract ：The objective of this study was to develop a macroporous resin adsorption method for separating and purifying aucubin in Eucommia ulmoides seed meal extracted into 80% aqueous ethanol solution. Twelve kinds of macroporous resins were compared for differences in their adsorption and desorption performances towards aucubin, and the results indicated that macroporous resins SIPI-7, HZ-820 and S-8 had better abilities to adsorb and desorb aucubin, among which, HZ-820 was considered to be the best resin due to the highest desorption rate. The optimal conditions for the adsorption and desorption of HZ-820 were found to be: sample loading, 0.5 g/g (weight ratio of