调整精卵相遇时间提高C57BL6J小鼠超排卵子受精率-中国实验动物学报.docVIP

研究报告 (调整精卵时间提高C57BL/6J小鼠超排受精率 曾文滔，张爱华，吕萌，刘燕 （南京医科大学江苏省医药动物实验基地，南京 210029） 【摘要】 目的 通过调整精卵时间，提高C57BL/6J小鼠超的受精率。方法 验证C57BL/6J鼠精能力；合笼后检栓，确定超排鼠交配时间，精卵相遇时间；将C57BL/6J雌鼠处理，停留6.5 h后配雄鼠，受精率，比较方法改进的效果。结果 表明使卵子高比例受精精子、卵子时间受精率；合笼后4 h雌鼠见栓，精卵7 h；根据结果，合笼时间推迟6 h，取受精卵22.27枚，比提高105%。结论： 调整精子、卵子时间可显著提高C57BL/6J小鼠超受精率。 【关键词】 C57BL/6J小鼠；超数排卵；精子；受精卵【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2013.012.000 To improve the fertilization rate of superovulated C57BL/6J mice by adjusting sperm and egg encounter time ZENG Wen-tao, ZHANG Ai-hua, LV Meng, LIU Yan (Jiangsu Animal Medical Experimental Center, Nanjing Medical University, Nanjing 210029, China) 【Abstract】Objective To explore the improving fertilization rate of superovulated C57BL/6J mice by adjusting sperm and egg encounter time. Methods Two hundred and forty-four 3-week-old female C57BL/6J mice and 8-week-old male C57BL/6J mice were used in this study. (I) To deterimine the fertilization rate by routine superovulation method, and use it as a control group. (II) To verify the insemination ability of C57BL/6J male mice. (III) To determine the time of coitus by checking vaginal plug at 3 time points after put the female and male into one cage, and to calculate the time for sperm and egg encounter. (IV) Female C57BL/6J mouse after superovulatory treatment, stayed for 6.5 h before mating with male mouse. To collect fertilized eggs, to calculate the fertilization rate and compare the effects of different treatment methods. Results (I) 10.88 fertilized eggs per mouse were collected in the control group; (II) In average 20.55 fertilized eggs per mouse were collected in the second group. It was 88.87% higher than that of the control group, indicating the ability of male sperm to get a higher fertilization rate of ova, and the sperm and egg encounter time was the key factor of fertilization rate. (III) For the majority of female mice showed va