7种鹤形目鸟类性别的分子鉴定_田秀华.pdf

　 ChineseJournal of Zoology 　2006, 41(5):62 ～ 67 7 ① ① ①② ①③ 田秀华 　刘　铸 　何相宝 　白素英 (①　　150040;②　　150040; ③　　150040) :, , 。 、。CHD 2550F 2718R, 7 :(Otis tarda)、(Grus japonensis)、(Anthropoides virgo)、(G.grus)、(G. leucogeranus)、(G.monacha)(Balearica regulorum) 48 , 。 7 , 。7 , 、、, 。 :;;CHD :Q75　:A　:0250-3263(2006)05-62-06 The Molecular Sexing of 7 Species of Gruiformes ① ① ①② ①③ TIAN Xiu-Hua 　LIU Zhu 　HE Xiang-Ba 　BAI Su-Ying (①Collegeof Wild Life Resources, Northeast orestry University, Harbin　150040; ②Harbin North orest Zoo, Harbin 　150040;③Centerfor Wild auna and lora, National Bureau of orestry Administration, Harbin　150040, China) Abstract:Fragmented ge graphical distributi n and illegal hunting have lead t endangered status f many bird spe- cies.Breeding pr grams in captivity and reintr ducti n f birds t their habitats are imp rtant f r pr tecting endangered species.Thus, it is imp rtant t identify the bird sex in rder t make c uples that can pr duce fertile eggs.Currently, it is difficult t identify the bird sex by a safe, c nvenient and accurate meth d.Sexes f 48 birds fr m 7 species f Gruif rmes [Great Bustard (Otis tarda ), Red-cr wned Crane(Grusjaponensis), Dem iselle Crane(Anthropoides vir- go), C mm n Crane(G.grus), Siberian White Crane(G.leucogeranus), H ded Crane(G.monacha), Grey Cr wned Crane(Balearica regulorum)] have been identified by primer 2550F 2718R (CHD gene ).This research n t nly makes breeding pr grams in captivity p ssible, but als will ffer assistance f r reintr ducti n f birds back t their habitats in the future.This meth d has sh wn its advantage as a safe, c nv