非洲猪瘟病毒P72蛋.pptVIP

Background of research African swine fever African swine fever (ASF) is a highly lethal viral hemorrhagic fever of domestic pigs. Both mortality and mobility of the virulent strain infection can be 100% with severe symptoms such as hemorrhage, breath difficulty and nervous disorder. The disease is listed as one of important class A diseases in animals by OIE and Chinese Ministry of Agriculture. Although China is free of the disease presently, it remains a big threat for Chinese pig industry with the increasing international trade and frequent people exchange. Therefore, it is urgent to establish quick accurate diagnostic methods for preventing the disease. Background of research Modality It is bursamembrane、symmetry with 20 sides、double-stranded DNA virus； Virus granules accumulate in cytolymph，average diameter is 200 nm ; Its center is a nuclear body alike with high density electron; The circumference is clear hexagon crust; The genomeis is about 170 ~210kb; The replicated location is intercytolymph. Background of research VP72 The vp72, together with vp32（phosphoprotein）、vp12（adsorption protein) and vp54（combine to LC8 chain of impetus protein）adsorbs and enters into cells. The vp72 is 32% with virus granules and is the major coat protein of ASFV, with conserved antigenicity ,has immunogenicity to natural infection ,and is often used as the target antigen for diagnostic of the disease. Background of research GST.Bind(TM) Resin GST.Bind(TM) Resin is an affinity chromatography support for rapid, single-step purification of recombinant glutathione S-transferase (GST) fusion proteins or native glutathione S-transferase or glutathione-binding proteins. The high degree of substitution ensures excellent binding capacity, allowing yields of 5–8 mg GST*Tag fusion protein per 1 ml settled resin. Aim Soluble expression and purification of fusion protein GST vp72S in E.coli Content Methods Mini Whole gel Elution GST affinity chromatography I