炎症因子IFN-γ和GM-CSF在EV-71相关重症手足口病早期诊断中的意义.docVIP

精品论文 参考文献 炎症因子IFN-γ和GM-CSF在EV-71相关重症手足口病早期诊断中的意义 王海彬 郭钧（通讯作者） 仇卫兵 胡利敏 　　(宿迁市泗阳县人民医院儿科 江苏 宿迁 223700) 　　【摘要】 目的:EV-71感染引起的手足口病发病率高，可引起神经系统等并发症，造成高死亡率。本研究拟探讨某些炎症因子与重症手足口的发生相关。方法:将79例患儿分为EV/CA/EV+CA/其他病毒感染组（A/B/C/D组），同时选用10例正常体检患儿作为对照组（E组）。通过RT-PCR方法检测受试者咽拭子及血清中EV-71、CA-16及其他病毒的核酸表达，通过酶联免疫吸附法检测血清中如白介素1beta;(IL-1beta;)、白介素6(IL-6)、白介素10(IL-10)、肿瘤坏死因子alpha;(TNF-alpha;)、集落刺激因子(GM-CSF)和干扰素gamma;(IFN-gamma;)分泌情况。结果:IL-1beta;,IL-6,IL-10和TNF-alpha;在A/B/C/D组内无明显差异；GM-CSF及IFN-gamma;在A组较B/C/D组明显升高。 结论:炎症因子IFN-gamma;和 GM-CSF可应用于由EV-71感染引起的手足口病的早期诊断。 　　【关键词】 EV-71；手足口病；炎症因子 　　【中图分类号】R725.1 【文献标识码】A 【文章编号】2095-1752（2016）09-0234-03 　　The significance of inflammatory cytokines IFN-gamma; and GM-CSF in the early diagnosis of severe HFMD effected by EV-71 virus 　　Wang Haibin,Guo Jun（Communication author）,Qiu Weibing,Hu Liming. 　　Department of pediatrics,Siyang Peoples Hospital,Jiangsu Shuqian,223700,China 　　【Abstract】Objective EV71 led to outbreaks of hand, foot, and mouth disease (HFMD) with severe mortality with a high rate of neurological complications. Our study was to investigate the relationship between inflammatory cytokines and severe HFMD induced by EV-71. Methods 79 patients were divided into A/B/C/D groups by the different viruses: EV / CA / EV + CA / other virus. We did the 10 election of healthy children as normal control group from physical examination. We used nasopharyngeal swabs and serums of participants to examine EV71, CA16, and other pathogens by RT- PCR kits. Cytokines of serum samples of participants were detected using ELISA kits. Results Levels of IL-1beta;, IL-6, IL-10 and TNF-alpha; have no significant difference among A/B/C/D groups inside; levels of GM-CSF and IFN-gamma; in A group are significantly higher than B/C/D groups. Conclusion Inflammatory cytokines IFN-gamma; and GM-CSF can be used for early diagnosis of HFMD infected by EV-71. 　　【Key words】EV71;HFMD;nflammatory factors 　　手足口病是由肠道病毒引起的发热出疹性疾病[1]。EV-71引起的多