微阵列第1次课.ppt

《微阵列设计与分析》第一章 绪论;微阵列技术起源;肿瘤基因组解剖计划;Once a Molecular Black Box;细胞的奥秘;疾病的进程;Genes Functional Regions Within DNA Molecules;Genes “Switch On”;Gene to mRNA to Protein;Cancer – Genes With Design Problems;Expression Profiles;Expression Profiles in Cancer;Measuring Expression Profiles Step 1. Isolating the mRNA;Measuring Expression Profiles Step 2. Converting mRNA into cDNA;Measuring Expression Profiles Step 3. Converting the cDNA into a cDNA Library;Measuring Expression Profiles Step 4. Isolating Each Bacterium;Measuring Expression Profiles Step 5. Identifying the Clones;Active Gene to EST;CGAP Helps Cancer Research in the Lab Gene Analysis and Gene Discovery;CGAP Helps Cancer Research in the Lab Understanding More About Cancer Biology I;CGAP Helps Cancer Research in the Lab Understanding More About Cancer Biology II;CGAP Helps Cancer Research in the Lab Understanding More About Cancer Biology III;CGAP Helps Cancer Research in the Lab Understanding More About Cancer Biology IV;CGAP Helps Cancer Research in the Clinic Understanding More About Cancer Biology;CGAP Helps Cancer Research in the Clinic Step 1. Microarrays in Diagnosis;CGAP Helps Cancer Research in the Clinic Step 2. Microarrays in Diagnosis;CGAP Helps Cancer Research in the Clinic Step 3. Microarrays in Diagnosis;CGAP Helps Cancer Research in the Clinic Differentiating Between Cancers with Similar Clinical Symptoms;CGAP Helps Scientists, Clinicians, and Patients;表达微阵列：同时检测成千上万个基因的表达值;微阵列的用途;1、药物筛选和新药开发 ;2、疾病诊断 ;又如对病原微生物感染诊断，目前的实验室诊断技术所需的时间比较长，检查也不全面，医生往往只能根据临床经验做出诊断，降低了诊断的准确率，如果在检查中应用基因芯片技术，医生在短时间内就能知道病人是哪种病原微生物感染；而且能测定病原体是否产生耐药性、对哪种抗生素产生耐药性、对哪种抗生素敏感等等，这样医生就能有的放矢地制定科学的治疗方案。;再如对具有高血压、糖尿病等疾病家族史的高危人群普查、接触毒化物质人群恶性肿瘤普查等等，如采用了基因芯片技术，立即就能得到可靠的结果，其他对心血管疾病、神经系统疾病、内分泌系统疾病、免疫性疾病、代谢性疾病等，如采用了基因芯片技术，其早期诊断率将大大提高，而误诊率会大大降低，同时有利于医生综合地了解各个系统的疾病状况。;3、环境保护 ;4、司法 ;5、现代农业 ;未来发展;本课程的适用范围