- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Cancer Gene Therapy (2007) 14, 968–975
r 2007 Nature Publishing Group All rights reserved 0929-1903/07 $30.00
/cgt
ORIGINAL ARTICLE
An archaeal histone-like protein mediates efficient p53 gene
transfer and facilitates its anti-cancer effect in vitro and in vivo
1 1 2 2 2 1 1 1
YY Li , R Wang , GL Zhang , YJ Zheng , P Zhu , ZM Zhang , XX Fang and Y Feng
1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University,
Changchun, China and 2Biotechnology Application Laboratory, Institute of Military Veterinary, Academy of
Military Medical Science, Changchun, China
The improvement of the transfection efficiency of the non-viral-based gene delivery systems is a key issue for the application in gene
therapy. We have previously described an archaeal histone-like protein-based (HPhA) gene delivery system and showed that HPhA
formed stable non-covalent complexes with nucleic acids and improved their delivery by using b-galactosidase as a reporter gene.
In this study, the wild-type p53 gene was transfected into the cancer cells using the HPhA as a vector, and the expression level and
the activity of p53 gene were evaluated both in vitro and in vivo. Gene expression was determined by real-time reverse
transcriptase-PCR and western blotting analysis. The cellular growth inhibition and apoptosis of HPhA-mediated p53 transfection
were assessed by XTT (sodium 30 -[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate)
assay and annexin V–FITC (fluorescein isothiocyanate) staining, respectively. Further more, transfection of HPhA/p53 into CNE
(nasopharyngeal carcinoma cell line)-xenografted nude
您可能关注的文档
- 制造自动化复习提纲(清华大学)【强推】戴晨旭整理笔记.pdf
- 制造自动化复习提纲(清华大学)chapter13.pdf
- 制造自动化复习提纲(清华大学)Chapter14.pdf
- 制造自动化复习提纲(清华大学)Chapter15.pdf
- 制造自动化复习提纲(清华大学)Chapter16.pdf
- 制造自动化复习提纲(清华大学)Chapter17.pdf
- 制造自动化复习提纲(清华大学)Chapter18.pdf
- 质量控制与质量管理(清华大学)00_01_Intro_Quality.pdf
- 质量控制与质量管理(清华大学)00_02_Intro_SixSigma_En.pdf
- 质量控制与质量管理(清华大学)01_01_7_Tools_En_V2.pdf
- 组蛋白与材料协同效应-Histone H3 Tail Peptides and Poly ethylenimine.pdf
- 组蛋白与材料协同效应-effect of PH on drug release.pdf
- 组蛋白与材料协同效应-p53 gene delivery system for tumor targeted transfection.pdf
- 组蛋白与材料协同效应-Poly L-glutamic Acid Grafted Polyethylenimine and Histone.pdf
- 组蛋白与材料协同效应-Synergistic effects between natural histone mixtures and polyethylenimine.pdf
- 组蛋白与材料协同效应-项目研究思路.doc
- 糖原代谢与分解Additional Pathways in Carbohydrate Metabolism.ppt
文档评论(0)