社区综合干预对高血压病患者的影响.docVIP

精品论文 参考文献 社区综合干预对高血压病患者的影响 宋贵禹1 严茂伟2 (1宜宾市第一人民医院药剂科 644000; 2成都中医药大学 611137） 【中图分类号】R96 【文献标识码】A 【文章编号】1672-5085(2010)22-0103-03 【摘要】 目的 建立安乐胶囊的质量标准。方法 采用薄层色谱(TLC)法鉴别处方中柴胡、甘草、钩藤、首乌藤四味药材；用高效液相色谱(HPLC)法测定甘草中甘草酸的含量，流动相为乙睛-0.2mol/L醋酸铵溶液-冰醋酸（25∶75∶0.3），检测波长250 nm，柱温30℃。结果 薄层色谱斑点清晰，阴性无干扰。甘草酸单铵盐进样量在0.032～0.416mu;g范围内与峰面积呈很好的线性关系，回归方程及相关系数：Y=1.1385times;106Xndash;0.036times;104，r=0.9999，加样回收平均回收率为101.2%，RSD=0.34%，表明回收率的重现性较好。结论 该法稳定，重复性好，可有效控制安乐胶囊的质量。 【关键词】 安乐胶囊 TLC HPLC 甘草酸 质量标准Study on Quality Standard of Anle Capsule Song guiyu,yan maowei(The No.1 peoples hospital of yibin,Zip 644000; Chengdu university of traditional Chinese medicine,Zip 611137) 【Abstract】 Objective To establish the quality standard of Anle capsules. Methods Raix Bupleuri, Radix Glycyrrhizae, Ramulus Uncariae Cum Uncis, Caulifs Polygoni Multiflori was identified by TLC. Glycyrrhizic acid was detected by HPLC. The HPLC condition of Glycyrrhizic acid was as foliows: the mobile phase consisted of acetonitrile-0.2mol/L Solution of ammonium acetate-glacial acetic acid(25:75:0.3), the UV detection wavelength was at 250 nm and the column temperature was 30℃. Results The characteristic identification by TLC was distinct and highly specific. The quantitative evaluation of Glycyrrhizic acid was linear within the range of 0.032～0.416mu;g, the regressive equation and correlative coefficient: Y=1.1385times;106Xndash;0.036times;104，r=0.9999, the average recovery was 101.2%(RSD=0.34%). Conclusion The method is exclusive, reproducible and suitable for the quality control of Anle Capsules． 【Key words】Anle Capsules TLC HPLC Quality standard 安乐胶囊系剂改品种，原处方来源于《卫生部药品标准》中药成方制剂第二册104页[标准编号：WS3-B-0274-90]，由柴胡、甘草、钩藤、首乌藤等八味药材组成。原标准中只有各类成分的理化鉴别，无各药味的薄层鉴别及含量测定项，为有效控制其质浚岣咧柿勘晖疲臼匝椴捎帽〔闵追ǘ圆窈⒏什荨⒐程佟⑹孜谔俣ㄐ约穑捎酶咝б合嗌追ǘ愿什葜懈什菟岬暮拷辛瞬舛ā?1 仪器与试药 岛津LC-10ATvp液相色谱仪（岛津公司），SPD-M10Avp二极管阵列检测器（岛津公司），SPD-10Avp紫外检测器（岛津公司），sartoriusBP211D电子天平（赛多利斯,感量0.1mg ；0.01mg。载量210g；80g）；autoscienceAS5150A超声波清洗器（昆