油菜抗咪唑啉酮类除草剂基因bnals1r等位基因特异pcr-作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2013, 39(10): 1711−1719 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.01711 油菜抗咪唑啉酮类除草剂基因BnALS1R 等位基因特异PCR 标记的开 发与应用 胡茂龙 龙卫华 高建芹 付三雄 陈 锋 周晓婴 彭 琦 张 维 浦惠明* 戚存扣 张洁夫 陈 松 / / , 210014 : BnALS1R M9 , BnALS1R BnALS1 1 SNP, 638 BnALS1R , , BnALS3 BnALS1 , 30 PCR (allele-specific PCR, AS-PCR), 3 AS-PCR F2BC1 BC2 PCR , 3 , 1211111, PCR , BnALS1R , 3 AS-PCR : ; ; BnALS1R ; ; PCR Development and Application of Allele-Specific PCR Markers for Imidazoli- none-Resistant Gene BnALS1R in Brassica napus HU Mao-Long, LONG Wei-Hua, GAO Jian-Qin, FU San-Xiong, CHEN Feng, ZHOU Xiao-Yin, PENG Qi, * ZHANG Wei, PU Hui-Ming , QI Cun-Kou, ZHANG Jie-Fu, and CHEN Song Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Sub-center, National Center of Oil Crops Improvement / Key Labo- ratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture, Nanjing 210014, China Abstract: A BnALS1R gene conferring resistance to the imidazolinone herbicides was previously isolated from an ALS (acetolac- tate synthase) inhabiting herbicide-resistant mutant line M9 in Brassica napus . A single nucleotide polymorphism leading to an amino acid substitution from serine to asparagine at site 638 of ALS was found between the BnALS1R in the mutant and BnALS1 in the wild type. We reported here the development of poly