Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut（Arachis hypogaea L.） Seeds研究.pdf

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Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut（Arachis hypogaea L.） Seeds研究.pdf

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Availableonlineatwww．sciencedirect，corn FunctionalAnalysisofthePhOsphOenOIpyruVateCarboxylaseontheLipid AccumulationofPeanut(ArachishypogaeaL．)Seeds PANLi-juan，YANGQing-li，CHIXiao-yuan，CHENMing-na，YANGZhen，CHENNa，WAN GTong，W G Mian，HEYa-nanandYUShan-lin ShandongPeanutResearchInstitute，Qingdao266100,P．R，China Phosphoenolpyruvatecarboxylase(PEPC；EC4．1．1．31)catalysesphosphoenolpyruvate(PEP)toyieldoxaloacetate，which isinvolvedinproteinbiosynthesis．Pyruvatekinase(PK；EC2．7．1．40)catalyzesPEPtoyieldpyruvate，whichisinvolved infatt acidsynthesis．Inthisstudy，fivePEPC genes AhPEPC1，AhPEPC2 AhPEPC3，AhPEPC4，andAhPEPC5 from peanuthavebeencloned．Usingaquantitativereal—timeIU1-PCRapproach，theexpressionpatternofeachgenewas monitoredduringtheseeddevelopmentoffourpeanutvarieties(El1，Hebeigaoyou，Naihan1，andHuayu26)．Itwas foundthatthesefivegenessharedsimilarexpressionbehaviorsoverthedevelopmentalstagesofEl1withhighexpression levelsat30and40dafterpegging(DAP)；whereasthesefivegenesshowedi~egulraexpressionpaRemsduringtheseed developmentofHebeigaoyou．InNaihan1andHuayu26，theexpressionlevelsofthefivegenesremainedrelativelyhigh inthefirststage．ThePEPC activitywasmonitoredduringtheseeddevelopmentoffourpeanutvarietiesandseedoil contentwasalsocharacterizedduringwholeperiodofseeddevelopment．ThePEPCactivityfollowedtheoilaccumulation patternduringtheearlystagesofdevelopmentbuttheyshowedasignificantlynegativecorrelationthereafter．These resultssuggestedthatPEPCmayplayaNimportantroleinlipidaccumulationduringtheseeddevelopmentoffourpeanut varietiestested． Keywords：peanut rachishypogaeaL．)，phosphoenolpyruvatecarboxylase，PEPCactiviyt，lipidaccumulation syntheticcarbonmetabolism (Cholleteta1．1996；Vidal lNTRODUCTlON andChollet1997)，andinplants，isgenerallyinvolved