il-18nf-κbinos在早期自然流产患者中的表达及其临床意义.docVIP

IL-18、NF-κB、iNOS在早期自然流产患者中的表达及其临床意义 陈一红1，陈丽红1，胡继芬1，陈虹2，陈余朋2 (1 福建医科大学附属第一医院 妇产科 350004，2福建医科大学附属第一医院 病理科350004) [摘要]目的 探讨白细胞介素-18 (Interleukin-18，IL-18)、核转录因子(Nuclear factor-kappa B，NF-κB)以及诱导型一氧化氮合酶(Inducible nitric oxide synthase，iNOS)的表达与早期自然流产的关系。方法 采用免疫组织化学SP法检测自然流产、正常早期妊娠患者外周绒毛及蜕膜中IL-18、NF-κB、iNOS的表达情况，用ELISA和硝酸酶还原法检测血清中IL-18和iNOS的含量。比较两组患者上述标本中L-18、NF-κB、iNOS的表达水平有无差别。结果 IL-18、NF-κB和iNOS在正常早期妊娠组和早期自然流产组的绒毛及蜕膜中均有表达。与正常早期妊娠组相比，早期自然流产组绒毛中IL-18、NF-κB和iNOS呈过度表达(P 0.05)，蜕膜中iNOS呈过度表达（P 0.05）。外周血中IL-18、iNOS的含量早期自然流产组明显高于正常早期妊娠组(P 0.05)。结论 IL-18、NF-KB和iNOS的过度表达可能与早期自然流产有关。外周血中IL-18、iNOS过渡表达可能是早期自然流产的预测分子。 [关键词] 早期自然流产；白细胞介素-18；核因子-κB；诱导型一氧化氮合酶 Clinical significance of interleukin-18, nuclear factor-kappa B and inducible nitric oxide synthase expression in patients with early spontaneous abortion Chen Yihong1，Chen Lihong1，Hu Jifen1，Chen Hong2, Chen Yupeng2 (1 Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University，Fuzhou 350004，China; 2 Department of Pathology, The First Affiliated Hospital of Fujian Medical University，Fuzhou, 350004, China) [Abstract] Objective To evaluate the relationship between IL-18,NF-κB and iNOS expression and ducidua in early spontaneous abortion. Method The expression of IL-18，NF-κB and iNOS of vili and ducidua were detected by S-P immunohistochemistry，The levels of IL-18 and iNOS in the supernatant were detected by enzyme-linked immunosorbent assay ( ELISA) and the nitric acid reductase method of the two groups. Result IL-18，NF-KB and iNOS can be detected in vili and ducidua in early spontaneous abortion and normal early pregnancy．Compared with normal early pregnancy group，the expression ofIL-18、NF-κB and iNOS in vili in early spontaneous abortion were distinctly increased (P0.05)； the expression of iNOS in ducidua in early spontaneous abortion was markedly increased(P0.05) ，the levels of IL-18 and iNOS of peripheral blood are distinctly increased (P0．05). Conclusion The overexpression of IL-18,