HPLC法检测人全血中西罗莫司的药物浓度研究.pdfVIP

HPLC 法检测人全血中西罗莫司的药物浓度 1 1 2 3 3 4 徐红冰 胡道德 陈桂良 谭建明 唐孝达 Ann gooding 1. 上海市第一人民医院药剂科 上海 200085 2. 上海市药品检验所 上海 200233 3. 上海市第一人民医院移植中心 上海200085 4. 美国惠氏制药公司 [摘要] 目的 建立全血中西罗莫司的测定法 方法 采用 HPLC 法 色谱柱为 YMC-Pack ODS-A 150mm 4.6 mm 5mI.D 预柱为Alltima C18 5 m Guard 7.5mm 4.6 mm 流动相为乙腈:四氢呋喃:水=55 :5 :40 检测波长为278 nm 流速为 1.5 ml/min 柱温 50 结果 其中西罗莫司及内标的保留时间分别约为 10.1 min 12.1 min 全血定量线性范围 2.49 76.36 ng/ml 最低检测浓度为 1.98 ng/ml 方法回收率为99.93 % 105.90 % 日内 日间RSD 小于7.40 % 结论 本法定量准确 适用于临床对西罗莫司的血药浓度监测 [关键词] 西罗莫司 色谱法 高压液相 药物浓度 Determination of the concentration of sirolimus in human blood by HPLC 1 1 2 3 3 4 Xu Hong-Bing , Hu Dao-De , Cheng Gui-Liang , Tan Jian-Ming , Tao Xiao-Da , Ann gooding (1.Department of Pharmacy, Shanghai first people’s hospital Shanghai 200080 China; 2 . Shanghai Institute for Drug Control Shanghai 200233 China; 3 . Transplantation Central, Shanghai first people’s hospital Shanghai 200080 China; 4. American-Wyeth Pharmaceutical Factory) [ABSTRACT] Objective:To monitor concentration of sirolimus in human blood. Methods:An HPLC method for the monitoring of sirolimus in human blood was performed on a column of YMC-Pack ODS-A (150 4.6 mm ID, 5m) with a guard column of Alltima C18,5m (Guard 7.54.6 mm), using a mixture of cetonitrile -tetrahydrofuran-H O (55:5:40) as mobile phase . 2 Sirolimus was detected at UV 278 nm and 32-desmethoxysirolimus was used as the internal standard. Results: The retention times of sirolimus and 32-desmethoxysirolimus were about 1