柱前衍生RP-HPLC法分析淡豆豉和大豆中氨基酸.PDFVIP

中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 9 期 2013 年 5 月 ·1199 · 柱前衍生 RP-HPLC 法分析淡豆豉和大豆中氨基酸 1 1* 2 李萍萍 ，崔元璐 ，蒋庆峰 1. 天津市现代中药重点实验室，天津中医药大学，天津 300193 2. 天津药物研究院，天津 300193 摘 要：目的 分析淡豆豉 Sojae Semen Preparatum 和大豆 Glycine max 中游离及水解氨基酸。方法 用 0.1 mol/L HCl 作溶 剂超声提取得到淡豆豉和大豆中的游离氨基酸；用 6 mol/L HCl 水解提取得到水解氨基酸，以异硫氰酸苯酯（PITC ）为柱前 衍生化试剂，采用柱前衍生 RP-HPLC 法进行分析。结果 淡豆豉和大豆中均含有 16 种氨基酸，这 16 种氨基酸浓度在 0.031～ 1.750 mmol/L 内呈良好的线性关系，r 均大于 0.997 9，平均回收率 91.02%～102.04%，RSD1.01%～4.81% 。结论 本方法灵 敏、准确，具有良好的重复性和稳定性，16 种氨基酸能在样品杂质量较多的情况下得到分离，且采用普通 C18 色谱柱成本低， 可广泛应用于富含氨基酸样品的分析。 关键词：氨基酸；淡豆豉；大豆；异硫氰酸苯酯；柱前衍生高效液相色谱 中图分类号：R286.022 文献标志码：A 文章编号：0253 - 2670(2013)09 - 1199 - 04 DOI: 10.7501/j.issn.0253-2670.2013.09.026 Analysis on amino acids in Sojae Semen Preparatrm and Glycine max by pre-column derivatization RP-HPLC 1 1 2 LI Ping-ping , CUI Yuan-lu , JIANG Qing-feng 1. Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China Abstract: Objective To analyze the free and hydrolyzed amino acids in Sojae Semen Preparatum (SSP) and Glycine max (GM) by pre-column derivatization RP-HPLC. Methods Free amino acids extracted in HCl (0.1 mol/L) by ultrasonic method and hydrolyzed amino acids extracted in HCl (6 mol/L) by hydrolysis method were both derivated by the agent phenylisothiocyanate so as to analyze them by gradient elution. Results There were 16 kinds of amino acids in both SSP and GM. The 16 kinds of amino acids had good linearity in the range of 0.031—1.750 μmol/mL with the coefficients of correlation all over 0.9979. The average recoveries were 91.02%—102.04% and the RSD values were between 1.01% and 4.81%. Conclusion The meth