流加发酵合成γ聚谷氨酸的研讨.pdfVIP

13 生物过程工程与控制 流加发酵合成γ-聚谷氨酸的研究 1 2 1 1 1 梁金丰,姚 俊,徐 虹,吴 群 ，张全景 1 2 (南京工业大学制药与生命科学学院, 江苏 南京210009； 南京医科大学基础医学院，江苏南京 210029 ) 摘要：通过发酵过程中流加葡萄糖和L-谷氨酸的流加发酵工艺实现了L-谷氨酸的高转化率以及γ-聚谷氨酸 -1 -1 -1 -1 产率的提高。实验结果表明，以0.8 g·L ·h 葡萄糖以及0.5 g·L ·h 的L-谷氨酸的速度补料发酵，γ- -1 -1 -1 聚谷氨酸的产量和生产强度分别为40.26 g·L 和0.42 g·L ·h ，并且L-谷氨酸的转化率高达95%。 关键词：γ-聚谷氨酸，补糖，补谷氨酸，流加发酵 Synthesis of γ-polyglutamic acid by fed-batch fermentation 1 2 1 1 1 Liang Jinfeng ,Yao Jun ,Xu Hong , Wu Qun , Zhang Quanjing 1 ( School of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, Jiangsu, China; 2 School of Basic Medical Sciences; Nanjing Medical University; Nanjing 210029; Jiangsu, China) ABSTRACT： A fed-batch culture was proposed by feeding of glucose and L-glutamate to achieve an efficient conversion of L-glutamate and enhance the γ-PGA production. The -1 -1 -1 -1 experimental results showed that at the feeding rate of 0.8 g·L ·h glucose and 0.5 g·L ·h -1 -1 -1 L-glutamate the yield and productivity of γ-PGA achieved 40.26 g·L and 0.42 g·L ·h , and the conversion rate of L-glutamate also reached a high level of 95%. KEYWORDS: γ-polyglutamic acid; glucose feeding; L-glutamate feeding; fed-batch fermentation; 引言 γ-聚谷氨酸(简称γ-PGA) 是由L-或/ 研究生.