基因工程原理Chapter 2.ppt

Chapter 2 Gene manipulation: an all-embracing technique;2-1 Introduction;New combinations of heritable material are constructed by the insertion of nucleic acid molecules, though virus, bacterial plasmid or other vector system outside cell, then the incorporated vector enters host organism and can multiply inside the host, showing unnatural. ;Simply speaking, gene manipulation permits stretches of DNA to be isolated (cloned) from their host organism and propagated in the same or a different host. ;2-2 Sequencing ;Scanning ;;Gene prediction;Promoter prediction;Deducing protein sequence;Motif: continuous sequence of amino acids or DNA so as to form a fragment. ;It is very difficult to predict a protein from three dimensional structure from aa motifs. However, compare more crystallographic data of proteins compare aa sequence of protein use computer summarize: a high degree of homology suggests similarity in function. ;2-3. In vivo biochemistry ;2-4. The new medicine;2-5. Biotechnology: the new industry;2-6. The central role of E. coli;A Genetic Ode 遗传赋(or A Melan Coli Tale,一个不起眼的大肠杆菌的述说);Then Lederberg and Tatum came and put me in the sun And watched me very closely to see what harm they’d done. Although they hadn’t killed me, they had really hurt my pride And though I looked quite normal I was quite upset inside. ;Next day they tried to feed me with my normal sort of food But they found I couldn’t use it in the way I always could. Glucose I could metabolize-in that I was proficient. But in synthesizing valine they soon found I was deficient.;They couldn’t find their valine so they went to biotin And till they thought just what to do they kept me dietin’. Then foresight and discernment made this lecturer and Prof. Enrich my food with Oxa cube and call me Oxo Troph. ;;;;;;The above poem was obtained from the Dept. of Bacteriology, Univ. of Wisconsin ;;人体生长激素释放激素(SS);E. coli;;;Homework