激光辅助孵化对冻融胚胎种植潜力的影响.pdfVIP

2012 NO.10 中外医疗 CHINA FOREIGN MEDICAL TREATMENT 论 著 激光辅助孵化对冻融胚胎种植潜力的影响① 刘吉 程东凯 李宝山 于洪君 孙晓玲 胡煜 宫立国 (沈阳东方医疗集团菁华医院生殖中心 沈阳 110005) 【摘要】目的 分析不同年龄段患者的胚胎冻融后进行激光辅助孵化对临床妊娠率的影响。方法 对713例患者,根据年龄、是否辅 助孵化、培养不同时间后行辅助孵化进行分组,比较各组间临床妊娠率。结果 ＜35岁的患者,2组临床妊娠率无差异;而＞34岁的患 者,临床妊娠率AH组高于非AH组,但无显著差异。复苏后立即对胚胎行辅助孵化再培养,相对于复苏后培养16～18h于移植前行辅助孵化 对提高临床妊娠率更有帮助,尤其对高龄患者,作用更明显(P0.05)。结论 辅助孵化技术的应用对冻融胚胎移植妊娠率的提升有一 定帮助,尤其是胚胎复苏后立即行辅助孵化能显著提高高龄患者的临床妊娠率。 【关键词】冻融胚胎 激光辅助孵化 临床妊娠率 【中图分类号】R714 【文献标识码 】Ａ 【文章编号】1674-0742(2012)04(a)-0023-03 Effests of Laser Assisted Hatching on Planting Potentiality in Frozen Thawed-embryo Transfer Cycles 【Abstract】Objective To analyze the influence of laser assisted hatching on clinical pregnancy rates in frozen thawed-embryo transfer (F-ET) cycles of different age patients.Methods A total of 713 F-ET cycles were included in this study.They were divided into different groups according to age, assisted hatching and the culture time,comparing with the clinical pregnancy rates.Results For the patients less than 34 years old, the clinical pregnancy rates of AH group and non-AH group is no difference;For the patients more than 34 years old, the clinical pregnancy rates of AH group is higher than non-AH group remarkable,but no significative difference.Comparing to hatching before embryo transfer, it is helpful to pregnancy hatching after embryo thawing immediately, especially for elder patients(P0.05).Conclusion Application of laser-assisted hatching on frozen-thawed embryo has certain significance to improve the clinical pregnancy rate,especially performing after thawing immediatel