毒理学相关资料,第七章内容参考文献9.pdfVIP

CMLS, Cell. Mol. Life Sci. 58 (2001) 356–370 1420-682X/01/030356-15 $ 1.50 + 0.20/0 © Birkhäuser Verlag, Basel, 2001 CMLS Cellular and Molecular Life Sciences Review Apoptotic and necrotic cell death induced by death domain receptors G. Denecker†, D. Vercammen, W. Declercq and P. Vandenabeele* Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, K. L. Ledeganckstraat 35, B-9000 Ghent (Belgium), Fax +32 09 2645348, e-mail: peter.vandenabeele@dmb.rug.ac.be Received 10 August 2000; received after revision 14 October 2000; accepted 6 October 2000 Abstract. Apoptosis and necrosis are two distinct forms death. Thus, the same cell death stimulus can result either of cell death. Caspases are indispensable as initiators and in apoptotic or necrotic cell death, depending on the avail- effectors of apoptotic cell death and are involved in many ability of activated caspase. Therefore, death domain of the morphological and biochemical features of apopto- receptors may initiate an active caspase-independent sis. Major changes in mitochondrial membrane integrity necrotic signaling pathway. In this review, we describe and release of proapoptotic factors, such as cytochrome c what is known about the apoptotic and necrotic cell from the mitochondrial intermembrane space, play an im- death pathways. Principal elements of necrosis include portant sensor and amplifying role during apoptotic cell mitochondrial oxidative phosphorylation, reactive oxy- death. In vitro studies of cell death in cell lines have re- gen production, and non-caspase proteolytic cascades vealed that inhibition of the classical caspase-dependent depending on serine proteases, calpain