結構生物學期末報告.pptVIP

結構生物學期末報告 生技所 一年級 鄭育欣 期末主題 @ 原因 : 因為TmAFP在阻止冰晶生長上比起魚類的AFP還有效100倍。所以我想找有關“ 海水魚”抗凍蛋白的結構和這篇結構加以比較。目的 : 從結構中是否可以看出為什麼昆蟲的AFP效率較好的可能原因。 @ TmAFP structure 中有水分子嗎?? @ TmAFP的多重序列比對 @ 抗凍蛋白會出現在人體中嗎?? TmAFP structure 尋找魚類的AFP 尋找魚類的AFP Structure Explorer - 1WFA @ Ice-binding structure and mechanism of an antifreeze protein from winter flounder. @ Nature 375 pp. 427 (1995) @ Sicheri, F., Yang, D. S. @ Pseudopleuronectes americanus 美國黃蓋鰈 Structure Explorer - 1UCS @ The Refined Crystal Structure of an Eel Pout Type III Antifreeze Protein Rd1 at 0.62-(Angstrom) Resolution Reveals Structural Microheterogeneity of Protein and Solvation @ Biophys.J. 84 pp. 1228 (2003) @ Ko, T.-P., Robinson, H., Gao, Y.-G., Cheng, C.-H. C., Devries, A. L., Wang, A. H.-J.: @ Lycodichthys dearborni 南極黏魚 PaAFP LdAFP LdAFP LdAFP LdAFP LdAFP 抗凍機制比較 抗凍機制比較 這些protein structures有水分子嗎?? TmAFP 1EZG:A THERMAL HYSTERESIS PROTEIN ISOFORM YL-1 QCTGGADCTSCTGACTGCGNCPNAVTCTNSQHCVKANTCTGSTDCNTAQTCTNSKDCFEA NTCTDSTNCYKATACTNSSGCPGH 1EZG:B THERMAL HYSTERESIS PROTEIN ISOFORM YL-1 QCTGGADCTSCTGACTGCGNCPNAVTCTNSQHCVKANTCTGSTDCNTAQTCTNSKDCFEA NTCTDSTNCYKATACTNSSGCPGH PaAFP 1WFA:A ANTIFREEZE PROTEIN ISOFORM HPLC6 (4 DEGREES C) - CHAIN A DTASDAAAAAALTAANAKAAAELTAANAAAAAAATARX 1WFA:B ANTIFREEZE PROTEIN ISOFORM HPLC6 (4 DEGREES C) - CHAIN B DTASDAAAAAALTAANAKAAAELTAANAAAAAAATARX LdAFP 1UCS:A ANTIFREEZE PEPTIDE RD1 NKASVVANQLIPINTALTLIMMKAEVVTPMGIPAEEIPKLVGMQVNRAVPLGTTLMPDMV KNYE Mutile sequence aligment 抗凍蛋白會出現在人體中嗎?? ~ Thanks ~ * *