膜性肾病综合征尿蛋白对肾小管上皮细胞凋亡的影响.DOCVIP

膜性肾病尿蛋白对人肾小管上皮细胞凋亡的影响 冯胜军1，吴丽丹2,3，刘华锋2 （1广东医学院基础医学院微生物与免疫学教研室，广东 东莞 523808；2广东医学院附属医院肾病研究所，广东 湛江 524023；3广东省东莞市石龙人民医院肾内科，广东 东莞 523326） [摘 要] 目的 初步探讨膜性肾病尿蛋白对人肾小管上皮细胞凋亡存在剂量依赖关系。方法 提取膜性肾病患者尿蛋白，进行蛋白成份分析、细菌内毒素试验）凋亡尿蛋白尿蛋白Effects of urinary protein in patients with membranous nephropathy on apoptosis of human renal tubular epithelial cells FENG Sheng-jun1, WU Li-dan2,3, LIU Hua-feng2 1Department of Microbiology＆ Immunology，Guang Dong Medical College，Dongguan 523808，China;2Institute of NephrologyGuangdong of Medical College, Zhanjiang 524001,China;3Nephrology department of Dongguan Shilong People’s Hospital, Dongguan 523326，China [Abstract]Objective：To study cell apoptosis on human renal tubular epithelial cells(hRTEC) induced by urinary protein from membranous nephropathy.Methods：Urinary protein in patients with membranous nephropathy was extracted, and analyzed of its for protein composition, bacterial endotoxin test and creatinine level. Test groups were different concentrations of urinary protein with 1.0mg/ml、2.0mg/ml、4.0 mg/ml、8.0 mg/ml stimulating hRTEC for 48h,control group was no protein. hRTEC apoptosis index were detected by Hoechst33258 dying method to determine the influence of different concentration of urinary protein concentrations on hRTEC induced apoptosis. Results：Urinary protein of membranous nephropathy were consisted of 56.4% albumin, 34.6%transferrin, 6.9%IgG.and its endotoxin and creatinine were below the lower limit of normal range of human. Test groups and the control group there was significant difference(p0.05), and the larger group and the smaller the concentration of urinary protein group significantly(p0.05). Conclusions：Between the apoptosis degree of human renal tubular epithelial cells and urinary protein concentration from in patients with membranous nephropathy existed dependent relationship. This study would further reveal mechanism and provide an important basis for the urinary protein how to bring about renal tubules-interstitial