连续流动氢化物发生原子吸收光谱法测定螺旋藻中痕量硒.pdfVIP

光谱学与光谱分析990431 光谱学与光谱分析 SPECTROSCOPY AND SPECTRAL peri.gif (2345 chinainfo.gif (4109 bytes) ANALYSIS bytes) 1999年 第19卷 第4期 Volume19 No.4 1999 连续流动氢化物发生-原子吸收光谱法 测定螺旋藻中痕量硒 刘波静　　钱利纯 摘　要　将被测试液、氢化元素-NaOH-NaBH溶液及HCl溶液用三道蠕动泵在相同速 4 率下，提取送入T型动态反应器，生成的气态氢化物由氩气载入等离子气中，测量谱线 强度，该方法不需分离，直接测定，灵敏度高，干扰少的特点，是测定痕量硒的一种 .－1 g ，样品回收率为98.89%～101.20%，结果令 有效分析手段，方法下限可达0.017 μg 人满意。 主题词　氢化物发生，　原子吸收光谱，　螺旋藻，　硒 Determination of Trace Selenium in Spirulina by Continuous-Flow Hydride Generation Atomic Absorption Spectrometry Bojing LIU and Lichun QIAN Institute of Feed Sci.Zhejiang Agricultural University ,310029　Hangzhou Abstract　The mixed solutions of the hydrogenized element-NaOH-NaBH4 and HCl solution were fed into hydride vapor generator at the same rate by multi-flow peristaltic pump.Selenium element in the aqueous solution reacted on the nascent hydrogen to generate gaseous hydride.No separation was needed and the method was highly sensitive with little interference.The selenium content in spirulina was determined and a reliable analytical . method was established.The determination limit of the method was 0.017 μg g-1,and the recovery of the samples was 98.89%—101.20%. Keywords　Hydride generation,　Atomic absorption spectrometry,　Spirulina,　Selenium 　　螺旋藻被誉为“21世纪最佳营养食品”具有广泛的开发前途，作为一种全营养食 品，其硒含量的测定占据着一定的重要地位。本文用连续流动氢化发生-原子吸收法测 定螺旋藻中硒含量，方法准确度、灵敏度和精确度均满足实际分析的要求，本文同时 讨论检测条件及共存元素对测定的干扰影响。 file:///F|/qk/gpxygpfx/gpxy99/gpxy9904/990431.htm（第 1／6 页）2009-12-22 10:53:33 光谱学与光谱分析990431 1　实验部分 1.1　仪器及装置 　　岛津AA-6501原子吸收光谱仪；HVG-1型氢化物发生器；国产硒空心阴极灯。