生长肥育猪的蛋白质与氨基酸营养PPT.pptVIP

Some Apparent Ileal Digestibilities, % 某些回肠表观消化率 Feedstuff 原料 Lysine Tryptophan Threonine Barley 大麦 73 73 70 Blood meal 血粉 81 -- 5 Canola meal 双低菜粕 75 67 -- Yellow corn 黄玉米 80 70 73 Fish meal 鱼粉 80 -- 76 Oats (grain) 燕麦 58 59 53 Peanut meal 花生粕 79 -- -- Sorghum grain 高粱 80 75 73 Soybean meal -Hi-pro 85 78 74 Soybean meal 豆粕 87 81 77 Sunflower meal 葵花粕 72 -- 71 Wheat 小麦 80 78 74 NRC, 1998 高蛋白豆粕 True Ileal Amino Acid Digestibilities 回肠氨基酸真实消化率 (Bellaver, 1989) Amino acid Corn Wheat Mids Soybean meal Cotton seed meal Poultry by-product meal Meat and bone meal Arg 94.3 94.9 96.5 92.8 89.8 88.2 His 78.5 73.9 86.5 79.3 77.4 74.5 Ile 78.9 72.5 87.4 73.6 80.6 74.6 Leu 83.3 67.3 84.2 72.1 78.6 74.4 Lys 59.9 60.0 86.1 59.7 78.1 75.9 SAA 86.2 75.9 91.8 83.0 81.7 79.2 Phe 84.5 78.1 87.9 84.1 80.1 78.3 Thr 71.7 59.6 82.9 71.5 74.1 69.1 Trp 68.4 73.3 82.8 73.9 61.5 60.8 Val 79.3 72.7 86.0 76.9 78.9 76.4 Lab Tests for Digestibility 消化率的实验室测定 FDNB - 2,4 flurodinitrobenzene (Carpenter method) FDNB binds with the epsilon amino group of lysine, if it is not bound to some other moiety, to form a color. It is assumed that non-bound amino nitrogen is correlated with higher digestibility. 2,4 氟二氮苯（卡本特法），FDNB与赖氨酸分子中未与其它基团结合的 o - 氨基结合，产生颜色反应。据认为未被结合的氨基氮与较高的消化率有关。 Pepsin digestibility - Incubate a sample of feed with pepsin and monitor nitrogen release. 胃蛋白酶消化率－将饲料样品与胃蛋白酶共同培养，检测氮的释放量。 Time, hours 时间，小时 Nitrogen release, % 氮释放，％ Lab Tests for Digestibility 消化率的实验室测定 Multienzyme assay Incubate sample with a mixture of peptidase, trypsin and chymotrypsin and monitor pH change over time. Liberated carboxyl groups release hydrogen ions resulting in a pH drop 复合酶法－将样品与肽酶，胰蛋白酶，胰凝乳蛋白酶共同培养 ，观察pH值随时间的变化。游离的羧基基团释放氢离子，导致pH下降。 Multienzyme pH True lysine Feed Ingredient change (units) digestibility Casein 酪蛋白 2.36 99 Soybean meal 豆粕 1.52 92 Poultry by-product禽副产品1.37 84 Meat meal 肉粉 1.37 85 Cottonseed meal