叶黄素_隐黄质及其异构体检测方法的研究_肖亚冬.pdf

Modern Food Science and Technology 2016, Vol.32, No.2 C30-HPLC-DAD β-APCI-MS 1.5%-MTBE -5:25:70V/V/V A1.5 %-MTBE -5:85:10V/V/V B0.6 mL/min24 min 20 μL 25 15-13/13’-9-9’-β- 15-13-13’-9-9’-β-2~150 ng 5~250 ng β- β- β- 1673-9078(2016)2-330-335 DOI: 10.13982/j.mfst.1673-9078.2016.2.048 Separation and Identification of Lutein,β-Cryptoxanthin and Their Isomers  XIAO Ya-dong1,2, LI Da-jing1,2, LIU Chun-quan1,2, ZHANG Zhong-yuan2 (1.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) (2.National Vegetable Processing Technology RD Sub-centers, Nanjing 210014, China) Abstract: A method to simultaneously detect all-trans-Lutein, all-trans-β-cryptoxanthin and their stereoisomers was developed using C30-column, high-performance liquid chromatography, diode array (C30-HPLC-DAD) technique. The structures of isolated compounds were elucidated by atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) and ultraviolet/visible (UV/Vis) spectroscopy. The optimal chromatographic condition was as follows: mobile phase A: 1.5% ammonium acetate:water:methyl tert-butyl ether (MTBE)-MeOH (5:25:70, V/V/V), mobile phase B: 1.5% ammonium acetate:water:MTBE-MeOH (5:85:10, V/V/V); flow rate: 0.6 mL/min; elution time: 24 min; injection volume: 20 μL; column temperature: 25 . Using this novel method, 15-cis-lutein, 13/13′-cis-lutein, 9-cis-lutein, 9′-cis-lutein, 15-cis-β-cryptoxanthin, 13- and 13′-cis-β-cryptoxanthin, 9-cis-β-cryptoxanthin and 9′-cis-β-cryptoxanthin were successfully separated from the mixture. The peak areas of all-trans lutein and all-trans β-cryptoxanthin showed a good linear relationship wi