英文-抗体工程.pdfVIP

serum polyclinic antibody generation cell engineering antibody 1980 3rd generation genes engineering antibody human-mouse chimeric antibody reshaped human antibody antibody fusion protein antibody library Antibodies are one of nature’s answers to the need for a versatile system to recognize and dispose of foreign organisms or substances. B cells, upon activation by antigen, differentiate into plasma cells, which secrete antibodies. All antibodies are categorized as immunoglobulins, B cell receptors are immunoglobulins Each immunoglobulins molecule is composed of four interlinked polypeptide chain. The two long chains are call heavy chains, and the short ones, light chains. The immunoglobulins have a “stem” called the Fc portion and comprising the lower half of the two heavy chains, each “prong” containing a single antigen-binding site. Thousand of mouse monoclonal antibodies have been produced from hybridomas over the past 30 years hetero-hybridoma Variable regions (from mouse) a chimeric antibody constant domain (from human Ig) (V region or complementarity- a humanized antibody determiningregions CDRs) Human monoclonal antibodies have been produced from human hybridomas for treatment The same technique can be used to clone human antibodies from transgenic mice Antibody engineering A recombinant fragments engineered for diagnostic and therapeutic application Pseudomonas aeruginosa, widespread multi-drug resistant vaccine inducedproduction of human anti Pa flagellin antibody or Lipopolysaccharide (LPS) or toxin A Human monocl