甘草黄连等五味中药对体外抗菌作用的研究-中国农学通报.PDFVIP

中国农学通报 2012,28(17):63-67 Chinese Agricultural Science Bulletin 甘草、黄连等五味中药对体外抗菌作用的研究 王春华，周铁忠，张正莹，王宏军 （辽宁医学院畜牧兽医学院，辽宁锦州121001） 摘 要：为验证清热解毒类中药体外联合抗菌协同指数(SI)判定标准的适用性。采用微管-平板法测定甘 草 (Radix glycyrrhiza)、黄连 (Rhizoma coptidis)、金银花 (Flos lonicerae j ap onicae)、黄柏 (Cortex phellodendri chinensis)和秦皮(Cortex fraxini) 的水提物和乙醇提取物对猪链球菌Ⅱ型的抗菌活性，用棋 盘微量肉汤稀释法测定这五味药不同提取物两两配伍的协同指数，然后将其组方，分别水煎煮和醇提 取，并测定其对猪链球菌Ⅱ型的最小抑菌浓度(MIC) 。结果表明：黄连和秦皮配伍SI 为0.5 ，甘草和秦 皮、甘草和金银花的SI 都为0.75 ，甘草和黄连组方后醇提物对猪链球菌Ⅱ型的MIC 为7.81 mg/mL ，甘草 和秦皮组方后醇提物对猪链球菌Ⅱ型的MIC 为1.95 mg/mL ，黄连和黄柏组方后的水提物对猪链球菌Ⅱ 型的MIC 为0.98 mg/mL ，黄连和秦皮组方后水提物对猪链球菌Ⅱ型的MIC 为1.95 mg/mL 。由此表明， 黄连醇提物和秦皮水提物的体外联合的协同作用明显，黄连和黄柏药对的水提物对猪链球菌Ⅱ型的抑 制作用较好。 关键词：中药；最小抑菌浓度；协同指数；猪链球菌Ⅱ型 中图分类号：S859.3 文献标志码：A 论文编号：2011-3847 TheStudyofAntibacterialActionofRadixGlycyrrhiza,Rhizomacoptidisetc FiveTraditionalChineseHerbsinVitro Wang Chunhua, Zhou Tiezhong, Zhang Zhengying, Wang Hongjun (DepartmentofVeterinaryMedicine,LiaoningMedicalUniversity, Jinzhou Liaoning 121001) Abstract: In order to validate the applicability of synergistic index (SI) decision criteria on mutual compatibility antibacterial activity of clearing heat and removing toxicity traditional Chinese medicine in vitro, antibacterial activity to streptococcus was detected by the microtube-flat method on water extract and ethanol extract from the Radix glycyrrhiza, Rhizoma coptidis, Flos lonicerae j aponicae, Cortex phellodendri chinensis and Cortex fraxini. SI was detected by the board trace broth dilution. SI was calculated by the formula. And then two Chinese herbs were matched into compound. The compound was extracted by water and ethanol solution. The minimu