真鲷天然抗性相关巨噬蛋白全长cdna的克隆与序列分析.pdfVIP

29 1 Vol. 29, No . 1 2005 2 JOURNAL OF FISHERIES OF CHINA Feb. , 2005 : 1000- 0615( 2005) 01- 0128- 05 # # cDNA 徐美瑜, 陈松林, 沙珍霞, 季相山 ( , 266071) : ; ; cDNA ; : S917 :A Cloning and sequence analysis of Nramp cDNA from Pagr us maj or XU Mei-Yu, CHEN Song-Lin, SHA Zhen-Xia, JI Xiang-Shan (Key Laboratoryf or Sustainable Utilization of Marine Fisheries Resources Certfi icated by the Ministry of Agriculture, Yellow Sea Fishery Research nstitute, Chinese A cademy of Fishery Sciences , Qingdao 266071, China) Abstract: Natural resistance associated macrophage protein ( Nramp) is an innate resistance protein to intracellular parasites, which is expressed plentifully in macrophage cells. Nramp has been studied in mouse, human, cattle, rainbow trout and channel catfish. However, little was known about the structure of Pagrus maj or Nramp. In order to get the complete sequence of Pagrus maj or Nramp, a pair of primer is designed according to a 200bp known sequence of Pagrus maj or Nramp cDNA. By the use of SMART RACE, the full Nramp of Pagrus maj or cDNA about 5 000 bp was obtained, including about 200 bp 5c terminal region ( UTR) , complete encoding region and c terminal region. There were ployA signals, which showed many possibilities of cutting at c terminal region. The character of Pagrus maj or Nramp nucleotide sequence and deduced amino acid sequence are analyzed. 12 putative transmembrane(TM) regions, a consensus transport motif ( CTM) , a predicted protein kinase C phosphroylation site and three predicted N-link glycosylation sites a