趋化因子mip-3α及其受体ccr6在溃疡性结肠炎中的表达及其意义.docVIP

CCL25/CCR9在溃疡性结肠炎黏膜中的表达研究 钟万锷1 曹萍1 许晶飞2 （1．315040，浙江省宁波市，宁波市第六医院消化内科；2．315040，浙江省宁波市，宁波市第六医院病理科） 【摘要】 目的： 研究趋化因子CCL25，CCL25的受体CCR9在溃疡性结肠炎（UC）中的表达情况以及与UC病变程度和范围的关系及相关性，探讨其在UC发病机制中的作用。方法： 用免疫组织化学法检测32例活动期UC及22例正常对照石蜡包埋组织中CCL25及CCR9的表达情况。结果： UC组CCL25及CCR9均为阳性表达。对照组为阴性或弱阳性表达。CCL25及CCR9在UC组的表达与对照组相比，差异有统计学意义（P＜0.01 ）。UC病变范围越广、程度越重，CCL25及CCR9表达阳性率越高，CCL25和CCR9表达具有相关性，（r＝0.759，（P＜0.01。 结论：CCL25/CCR9相互作用参与了UC的发生和发展。CCL25/CCR9高低反映了疾病的炎症程度，且可能作为药物治疗的潜在靶点。 【关键词】溃疡性结肠炎；CCL25； CCR9；免疫组织化学 Expression of CCL25/ CCR9 in ulcerative colitis ZHONG Wan-e1, CAO Ping1，XU Jing-fei2(1. Department of Gastroenterology,Ningbo No.6 Hospital, Ningbo, 315040,china; 2. Department of Pathology, Ningbo No.6 Hospital, Ningbo, 315040,china.) 【Abstract】 ObjectiveTo investigate the role and the expression of chemokine CCL25and its receptor CCR9 in ulcerative colitis(UC) and to explore the relationship between CCL25 and CCR9 and the severity of disease. Methods: The level of CCL25 and CCR9 in colon mucosa of UC patients was detected by immunohistochemistry method. Results: The level of CCL25 and CCR9 in colon mucosa of UC patients were much higher than that of normal controls. The expression of CCL25 and CCR9 was related to the extent of lesion and the degree of disease. The expression of CCL25 was significantly correlated with that of CCR9（r=0.759，P＜0.01). conclusions: The expression of CCL25 was correlated with that of CCR9, CCL25and CCR9 are closely related with the occurrence and development of UC. The expression of CCL25 and CCR9 reflect the degree of inflammation of UC, and suggesting that CCL25 or CCR9 antagonism may represent a relevant pharmacological target for the design of novel pharmacological treatments in human UC. 【Key Words】lcerative colitis; CCL25; CCR9; immunohistochemistry 溃疡性结肠炎 ( UC) 是一种病因发病机制未明的。目前认为其发病可能与遗传、免疫、感染及精神等多因素有关而免疫学因素是 UC 研究中的热点。免疫学因素细胞因子细胞因子分为三类促炎细胞因子、抗炎细胞因子以及生长因子。促炎细胞因子与抗炎细胞因子之间的平衡失调UC 的一个重要的发病机制CCL25/CCR9在UC中的表达情况以及与病变程度的关系，探讨CCL25/CCR9在UC发病中的作用。 1 材