同步辐射元素成像技术PPT.pptVIP

同步辐射元素成像技术在环境科学 研究中的应用;元素成像：样品内元素的空间分布 同步辐射：电子运动的方向发生变化时在切线方向产生电磁辐射 1947年, 在电子同步加速器上首次观察到，命名为 synchrotron radiation ;同步辐射的特点;;;元素成像原理 ;元素成像原理 ;同步辐射元素成像设备 ;成像设备;XRF spectra;生物样品中微量元素的空间分布;1 mm;Cyclotella meneghiniana account for 20% of global carbon fixation. facilitate the transport of photosynthetically fixed CO2 from the surface ocean to deep water , 10μm 制样 细胞悬液滴加在电镜栅网上，自然风干 定量 XRF standards NIST 1832 ,1833;XRF microprobe at beamline 2-ID-E of the Advanced Photon Source Following monochromation the X-ray beam was focused to a spot of approximately 270?nm diameter using a Fresnel zone-plate objective. Fluorescence spectra were recorded at 150?nm intervals as the specimen was raster scanned through the focus of the X-ray beam to obtain 2D projection images. The flux transmitted by the sample was monitored using both an ion chamber and a configured detector. A rotation series was obtained by acquiring such images with the specimen oriented at 24 different angles with respect to a vertical rotation axis. ;Quantitative 3D elemental microtomography of Cyclotella meneghiniana at 400-nm resolution PNAS, 2010, 107, 15676-15680;各元素分布图叠加PNAS, 2010, 107, 15676-15680; C. meneghinianas三维结构的元素分布细节PNAS, 2010, 107, 15676-15680;实测值和重建数据（36.0°）;XAS 成像;Spot size 15 or 5 μm diameter focused by tapered metal monocapillaries 鲜活组织，室温，潮湿 定量：0.2μL 5 mM Arsenate滴于滤纸;Localizing the Biochemical Transformations of Arsenate in a Hyperaccumulating Fern;EST, 2006, 40, 5010-5014;EST, 2006, 40, 5010-5014;方法评价;; Detection of mercury-, arsenic-, and selenium-containing proteins in fish liver from a mercury polluted area of Guizhou Province, China with SRXRF after IEF;Imaging Metals in Proteins after native Electrophoresis;Detection of Se-containing proteins in selenium-enriched yeast with SRXRF after 2-dimensional gel electrophoresis ;Metallome of Nile tilapia tissue identified by SRXRF after 2Dseparation;准在线联用技术 非破坏性、基底影响小 同时给出蛋白质表达水平、等电点、分子量、所含金