医学论文-和血益智方对 VD 大鼠学习记忆和海马的影响.docVIP

医学论文-和血益智方对 VD 大鼠学习记忆和海马的影响 ???????????????????? 作者：田先翔 梅琼彬 王立 袁芳 袁尚荣 吴正治? 【摘要】? 目的观察和血益智方对 VD 大 鼠行为学和形态学的影响。方法动物随机分为假手术组和模型组。VD 模型制作成功后随机分为和血益智方小剂量组和大剂量组、思尔明组、生理盐水（NS）组。用穿梭箱和水迷宫检测大鼠行为学并作海马 HE 常规染色，光镜观察。结果和血益智方、思尔明均能缩短VD 大鼠穿梭箱实验主动逃避时间和遭受电击时间，减少遭受电击次数；能缩短 VD 大鼠水迷宫实验时定位航行潜伏期、游泳的路径，增加大鼠空间探索实验中穿越原目标的次数。NS 组海马细胞体积变大、核变小、有核仁消失和核固缩；除 假手术组正常外，其余各组海马细胞层次有减少，但细胞排列比较密集、细胞形态尚可。结论 和血益智方可 改善 VD 大鼠的学习记忆能力。 【关键词】? 血管性痴呆；MORRIS 水迷宫；学习记忆；海马 ??? 〔Abstract〕 Objective???? To? study? the? effects? of? Hexue? Yizhi? Formula? on? learning? and? memory? and? the? morphology? of? the? hippocampal? neurons? of? the? rats? with? Vascular? Dementia? (VD).??? Methods???? The? rats? were? subjected for? ischemia-reperfusion? repeatedly? by? blocking? the? bilateral? common? carotid? arteries? to? establish? VD? models.? They were? divided? into? four? groups? randomly? after? the? establishment? of? VD? model.? The? treating? groups? were? administrated with? Hexue? Yizhi? Formula? (1? mL/100? g)、Hexue? Yizhi? Formula? (2? mL/100? g)、 Sermion、Natural? Salt? Solution? (NS)? respectively.? Animals? with? the? shamed-operation? were? taken? as? control.? The? behavior? changes? were? observed through? the? Shuttle? box? test? and? water? maze? test? at? the? end? of? treatment.??? Results???? In? shuttle? box? test? Stimulus? Frequency? (SC) 、Stimulated? Time? (ST)? and? Active? avoidance? Response? Time? (ART)? of? the? rats? were? reduced? respectively? in? Sermion、Hexue? Yizhi? Formula? (1? mL/100? g)、Hexue? Yizhi? Formula? (2? mL/100? g)?? groups? than? those in? NS? group.? In? the? Morris? water? maze? test,? Stage? of? Latency? and?? Pathway? were? declined? and? the? times? were? increased?? markedly? acrossing? the? target.? The? structure? levels? of? hippocampal? neurons? reduced,? but? cells? were? more? normal? and intensive? in? treated? groups? except? NS? group.??? Conclusion???? Our? study? suggest? that? Hexue? Yizhi? Formula? can? improve? the? ability? of? the? leaning? and? mem