医学论文-大鼠退变腰椎间盘组织的超微结构观察.doc

医学论文-大鼠退变腰椎间盘组织的超微结构观察 【摘要】? 　　［目的］观察大鼠腰椎间盘退变动物模型中椎间盘组织的超微结构改变。［方法］32只SD大鼠随机分为实验组和对照组，每组各16只。实验组采用手术方法以L3为中心切除棘突、关节突、棘上、棘间韧带，切断双侧竖棘肌。对照组仅切开皮肤后即缝合。术后8周，应用电镜技术对SD大鼠椎间盘组织进行详细的超微结构观察。［结果］对照组的椎间盘组织超微结构的病理改变不显著，而实验组表现为软骨样细胞减少，出现不同程度地退变、坏死，细胞器数目减少，细胞外周致密颗粒增多；基质中胶原纤维发生不同程度的变性、融合、扭结或钙化，胶原纤维束间裂隙增大；［结论］应用电镜观察腰椎间盘退变动物模型的超微病理改变，可为研究腰椎间盘疾患提供相关的实验依据。 【关键词】? 椎间盘退变 超微结构 动物模型 　　Observation of ultrapathology in the rat model study of experimental lumbar intervertebral disc degeneration∥??? 　　Abstract：［Objective］To observe the change of ultrapathology in the rat model study of experimental lumbar intervertebral disc degeneration.［Method］Thirtytwo SpragueDawley（SD）rats were divided into two guoups at random,including in the experimental group and the control group.The experimental group was incised through posterior approach along spinous process,resected below erector spinal muscle,the supraspinal and interspinal ligament and the posterior of the zygapophysial joint at the center of the third lumbar spine for providing the rat degeneration of lumbar intervertebral induced by mechanical instability state.The control group was just incised the skin.All of th rats were examined after eight weeks by electronmicroscope.［Result］There was no special ultrapathological characterization after operation in the control group,while in experimented group most chonduocytelike cells were degenerated or necrotic and their number decreased markedly,collagenous fibrils appeared various kinds of degeneration including fusion,twist and calcification,and there were gaps between the collagen bundles.［Conclusion］Observing the change in the rat model study of experimental lumbar intervertebral disc degeneration by ultrapathology,it can offer the experimental data studying tile degeneration of lumbar intervertebral disc.??? 　　Key words：intervertebral disc degeneration;? ultrapathology;? animal model 　　椎间盘退变是腰腿痛和下腰痛的重要原因之一，也是腰椎间盘突出症和其他腰椎疾患的病理基础。但椎间盘发生退变的机理尚未完全阐明，本文作者通过设计和建立一种新的腰椎间盘退变的动物模型，并从退变的腰椎间盘髓核组织中观