医学论文-八角茴香药用成分的提取及其镇痛作用的实验研究.docVIP

医学论文-八角茴香药用成分的提取及其镇痛作用的实验研究 ?????????? 作者：林洁，兰琪欣，韦应芳，廖兰艳，韦国锋?? 【摘要】? 目的 研究八角茴香药用成分的提取工艺及其对小鼠的镇痛作用。方法 采用乙醇提取、乙酸乙酯等有机溶剂萃取八角茴香，经大孔吸附树脂柱分离，得到白色精细粉末的莽草酸，并对莽草酸进行化学定性分析，镇痛实验采用扭体法和温浴法，观察腹腔注射莽草酸溶液（莽草酸组）后小鼠痛阈潜伏期和扭体反应的次数，设阿司匹林和生理盐水为对照组。结果 八角茴香含有莽草酸成分，其提取物对扭体法致小鼠疼痛的镇痛作用与生理盐水组比较差异有显著性（P0.05）；在给药后20min、60min痛阈潜伏期也比生理盐水组明显延长(P0.05），但与阿司匹林组比较差异无显著性（P0.05）。结论 八角茴香中含有莽草酸，对小鼠具有明显的镇痛作用。 【关键词】? 八角茴香；植物提取物；镇痛；小鼠；动物实验 　　Abstract:? Objective?? To investigate the procedures of extracting medicinal components of star anise and its analgesia on mice.? Methods? Star anise were extracted from Illicium verum? Hook.f. using ethanol and ethyl acetate as solvents. After macron absorption resin layer separation, a kind of white fine powder shikimic acid were obtained.? The extracted shikimic acid were determined for chemical qualitative analysis. Writhing test and warm bathing test were performed and observed the mice pain threshold latency and writhing reaction times after giving peritoneal shikimic acid extraction solutions (shikimic acid group). Aspirin and? normal saline were used as controls.? Results? The shikimic acid components can be obtained from star anise. Compared to normal saline group, the shikimic acid group had better analgesic function (P0.05), 20 minutes and? 60 minutes after drug administration pain threshold also prolonged ( P0.05), but compared to Aspirin group, there was no statistical significant difference (P0.05).? Conclusion? Satar anise contains? shikimic acid which has effective analgesia on mice.? ??? 　　Key words:? Anise, star; plant extracts; analgesia; mice; animal experiment 　　八角茴香为木兰科植物八角茴香lllicium verurm Hook.f.的果实，其味辛、甘、温，入脾、肾经，具有温阳、散寒、理气之功效。主治中寒呕逆，寒疝腹痛，肾虚腰痛，干温脚气［1］。由于资源丰富，具有较强的药理作用，深受人们的青睐。近年来的研究表明［2］，莽草酸具有抑菌、升高白细胞、镇痛作用以及其他茴香醚具有雌激素样作用和较强致敏作用。笔者主要对八角茴香镇痛成分的提取工艺及对小鼠的镇痛作用进行研究。 　　1? 材料与方法 　　1.1? 实验材料? 八角茴香：采自广西德保县。动物：小白鼠由本院实验动物中心提供。仪器：电热恒温水浴锅，北京长原实验仪器厂；秒表，上海秒表厂；鼠筒，自制；2FA02-旋转蒸发仪，上海玻璃仪器；层析柱。试剂：乙醇；阿司匹林片，广西南宁制药正业集团公司生产；醋酸；丙酮；乙酸乙酯；生理盐水；甲醇。 　　1.2? 实验方法 　　1.2.1? 八角茴香中莽