医学论文-人类 HLA-DP 基因多态性与溃疡性结肠炎相关性研究.docVIP

医学论文-人类 HLA-DP 基因多态性与溃疡性结肠炎相关性研究 ??????????????? 作者：郭海建 刘若丹 周向京 刘新民 刘俊 熊鹰 王建平 胡银清 【摘要】? 目的：从基因水平探讨溃疡性结肠炎（UC）的发病机制，并分析其与抗中性粒细胞胞浆抗体（ANCA）及疾病分型的关系。方法：用间接免疫荧光法分 别对 86 例 UC 患者和128 例健康者进行血清 ANCA 检测 ，同时应用聚合酶链反应-限制性片段长度多态性核苷酸分型技术检测深圳汉族人 HLA-DPB1 基因多态性，用 PCR 技术对 HLA-DPB1 基因第 2 外显子（exon 2）进 行体外扩增 ；然后用 Ban II、Fok I、Dde I、Rsa I、EcoN I、BstUI 6 种限制性内切酶对扩增产物进行酶切，电泳分离酶切片段 ，根据片段格局 确定相应基因型别。结果：UC 患者 ANCA 的阳性率为 53.5%，对照组均为阴性；UC 患者 HLA-DPA1 等位基因频率（0.49）与对照组（0.18）比较 ，差异有 显著性（P = 0.0032，RR = 5.681， Pc = 0.033)。结论：HLA-DPA1 和 ANCA 在 UC 中阳性率均显著增加 ， HLA-DPA1 与溃疡性结肠炎有一定关联 ，溃疡 性结肠炎患者与正常人之间存在着免疫遗传异质性的差异。 【关键词】? 溃疡性结肠炎；抗中性粒细胞胞浆抗体；HLA-DPA1 基因；聚合酶链反应；限制性片段长度多态性 ??? 〔Abstract〕??? Objective???? To? study? HLA-DPA1? genotyping? and? to? analyze? the? relationship? among? HLA-DP? genotyping、antineutrophil? oplasmic? antibodies? (ANCA)、clinical? classification? of? ulcerative? colitris? (UC).?? Methods? Eighty-six? UC? patients,? 128? healthy? subjects? (HS)? were? enrolled,? and? serum? ANCA? with? indirect? immunoflorecence? and? HLA-DPAl? genotypes? were? examined? in? 86? patients? with? ulcerative? colitis? and 128? healthy? controls.? HLA-DPAl? typing? was? carried? out? by? digesting? the? locus? specific? polymerase? chain reaction? amplified? products? with? alleles? specific? restriction? enzymes? (PCR-RFLP),? Ban? II,? Fok? I,? Dde? I, Rsa? I,? EcoN? I,? BstU? I.?? Results????? Positive? rate? of? ANCA? in? UC? patients? was? 53.5%.? But? 0.0%? in? HS. The? allele? frequency? of? DPA1? genotyped? by? polymerase? chain? reaction? with? sequence? specific? primers. When? UC? patients? were? divided? into? ANCA? positive? and? negative? groups,? it? is? found? that? DPA? gene? positive? rate? was? significandy? higher.??? Conclusion???? In? China? the? positive? rate? of? HLA-DPA1? and? ANCA? is significantly? increasing? in? patients? with? ulcerative? colitis. ??? 〔Key? Words〕???? Ulcerative? Colitis ;? ANCA? HLA-DPAl? gene;? Polymerase? chain? reaction;? Restrictedfragment length? polymorph