医学论文-芍药苷对LPS诱导的RAW264.7细胞HMGB1表达的影响.docVIP

医学论文-芍药苷对LPS诱导的RAW264.7细胞HMGB1表达的影响 ???????????????????? 作者：常蕴青 赵中夫 刘明社 【摘要】? 目的：观察芍药苷对脂多糖(LPS)诱导的小鼠单核/巨噬细胞株(RAW264.7)高迁移率族蛋白B1（HMGB1）释放和表达的影响。方法：体外培养的小鼠腹腔巨噬细胞培养三代后，转种于放置了无菌处理盖玻片的6孔培养板中，将细胞分为对照组、LPS刺激组（100 ng/mL）、芍药苷组（LPS 100 ng/mL＋芍药苷0.625 mg/mL）；LPS刺激20 h后，免疫细胞化学法检测各组培养细胞HMGB1的表达水平。结果：LPS刺激RAW 264.7细胞20 h后，LPS组细胞核HMGB1特异性染色变淡，胞浆染色加深，表明HMGB1从胞核转移到胞浆; 芍药苷组大部分细胞核HMGB1特异性染色深，而胞浆染色浅，表明芍药苷抑制HMGB1从胞核转移到胞浆。对HMGB1免疫组化数字图像的累积光密度值分析显示，HMGB1含量在三个实验组间差异有统计学意义（F=918.67，P0.01），LPS刺激组和芍药苷组间差异有统计学意义（q=22.66,P0.01）。结论：芍药苷可能通过抑制HMGB1的核转位和释放，对LPS诱导的肝损伤起保护作用。 【关键词】? 芍药苷；脂多糖；小鼠腹腔巨噬细胞；高迁移率族蛋白B1 ??? Abstract? Objective：To investigate the effects of paeoniflorin on the expression of high mobility group box1(HMGB1) in RAW264.7 macrophages induced by lipopolysaccharide (LPS). Methods: RAW264.7 Macrophages were cultured to the third generation in six wells plate and divided into three groups : control group, 100 ng/mL LPS treatment group and 0.625 mg/mL paeoniflorin treatment group. After 20h treatment, the expression of HMGB1 was measured by immunohistochemical method . Results: At 20 h after RAW264.7 cells stimulated by LPS, LPS group showed nucleus HMGB1-specific stained light, cytoplasm stained deepened. It was indicated that HMGB1 was transfered from the nucleus to the cytoplasm. Paeoniflorin group showed the release of HMGB1 of? the majority of cells was inhibited. HMGB1-specific staining nuclei became darker. cytoplasm staining shallow. Integrated optical density of HMGB1 immunohistochemic digital image analysis showed that HMGB1 among three groups were significantly different (F=918.67，P0.01). The difference between LPS group and Paeoniflorin groups was statistical significance（q=22.66,P0.01）. Conclusion: Paeoniflorin can protect liver injury under LPS stimulation through inhibiting the tranfer and release of HMGB1. ??? Key words? Paeoniflorin;Lipopolysaccharide;RAW264.7 macrophages;High mobility group box 1(HMGB1) ??? 芍药苷(paeoniflorin，Pae) 为一种蒎烷单萜苷，主要存在于毛莨科植物芍药（Paeonia lactiflora ?