医学论文-归元创疗汤剂对创伤大鼠血浆内毒素及二胺氧化酶的影响.docVIP

医学论文-归元创疗汤剂对创伤大鼠血浆内毒素及二胺氧化酶的影响 【摘要】? 目的：通过中药方剂归元创疗汤对闭合性创伤大鼠模型的治疗，研究中药对血浆内毒素及二胺氧化酶的影响。方法：Wistar 大鼠 54 只，随机分成：正常组、中药组、对照组，其中中药组和对照组为创伤模型，分别给大鼠予灌胃归元创疗汤或生理盐水治疗，在不同时段进行血浆内毒素以及二胺氧化酶测定。结果：创伤大鼠的血浆内毒素及二胺氧化 酶明显高于正常大鼠，但中药组大鼠的血浆内毒素及二胺氧化酶低于对照组。结论：归元创疗方剂在早期创伤治疗中，具有保护胃肠黏膜屏障、抑制细菌内毒素移位作用，可减轻炎症反应。 【关键词】? 创伤大鼠；归元创疗汤；内毒素；二胺氧化酶 ??? 〔Abstract〕??? Objective???? Therapy? of? rats? suffered? from? closed? trauma? with? Guiyuan? Chuangliao? Decoction,? to? study? the? anti-inflammatory? effects? and? mechanism? of? Chinese? Medicine.??? Methods? 54? Wistar? rats? were? divided? into? three? groups? at? random:? normal? group? with? no? trauma? and? therapy;? Chinese? medicine? group? with? trauma? received? gastric? infusion? of? Guiyuan? Chuangliao? Decoction;? Control? group? with? trauma? received? gastric?? infusion? of? saline.? Other? measure? was? completely? identical? in? last? two? groups.? Levels? of? endotoxin? and? the? activity? of? diamine? oxidase? (DAO)? in? the? plasma? were? measured? in? order? to? determine? the? effect? of? Guiyuan? Chuangliao?? Decoction.??? Results???? The? levels? of? plasma? endotoxin? and? DAO? in? traumatic? rats? were? significantly? higher? than? those? of? normal? group.? The? levels? of? plasma? endotoxin? and? activity? of? DAO? were? significantly? lower? in? Chinese medicine? group? than? those? in? control? group.??? Conclusion???? These? results? indicated? that? Guiyuan? Chuangliao Decoction? taken? orally? can? abate? the? degree? of? intestine? injury,? restrain? the? endotoxin? and? pathogenic? bacteria? to translocate? into? systemic? organs? and? systemic? circulation,? may? lessen? the? systemic? inflammatory? response. ??? 〔Key? Words〕??? Traumatized? rat;? Guiyuan? Chuangliao? Decoction;? Endotoxin;? Diamine? oxidase? (DAO) ??? 自上世纪 80 年代以来，人们注意到机体最大的细菌及毒素贮库——肠道可能是原因不明感染的“策源地”，肠道细菌和内毒素移位所致的肠源性感染与严重创伤等应激后发生的脓毒症、多器官功能障碍密切相关。目前认为：胃肠道是脓毒症和多器官功能障碍综合征的启动机[1]。本研究以创伤大鼠为模型，观察归元创疗汤在创伤早期通过改善和保护胃肠黏膜、抑制细菌内毒素移位，达到对机体保护作用。 ??? 1??? 材料和方法 ??? 1