梭鱼和青鳉鱼ERα介导的雌激素活性比较研究 Comparison of ERα-mediated estrogenic binding activity between So-iuy mullet (Mugil soiuy) and medaka.pdfVIP
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梭鱼和青鳉鱼ERα介导的雌激素活性比较研究 Comparison of ERα-mediated estrogenic binding activity between So-iuy mullet (Mugil soiuy) and medaka
EnvironmentalScience
1):1490--1495 China
中国环境科学2010,30(1
梭鱼和青鲔鱼ERa介导的雌激素活性比较研究
郝丽妮地,赵砚彬2,彭 辉2,黄 崇2,陈树林1,胡建英2‘(1.西北农林科技大学动物医学院,陕西杨凌
712100:2.北京大学城市与环境学院,北京100871)
长1113
bp的梭鱼ERa(sERa)DEF区片段,并以此构建pGBT9一sERa
3.3倍,说明梭鱼ERa对El、E2、E3、EE2具有较高的敏感性。这为解释辽东湾梭鱼雌雄同体的高发生现象提供了重要线索.
关键词:ERa:梭鱼:酵母双杂交测试;物种敏感性
中图分类号:R994.6 文献标识码:A 文章编号:1000-6923(2010)11—1490—06
ofERa-mediated between
Comparison estrogenicbindingactivity So-iuymullet(MugUsoiuy)and
ofAnimal
Medicine,
Li—niL2,ZHAOYah-Bin2,PENGHui2,HUANG Shu·Linl,HUJian-yin92’(College
Chon92,CHEN
of andEnvironmental
NorthwestAF Urban
712100,China:2.Department
University,Yangling
100871,China).China
University,Beijing
thewild mulletof has ofintersexfrom
Abstract:Toa reason frequency
possiblewhy So—iuy LiaodongBay high
explore
view ERa eDNA of111 3
the of of
point specie—specificbindingsensitivity,afragmentbpencodingSo—iuy
DEFdomainwas andusedtoconstruct DEFwas
estrogenreceptor amplified plasmidpGBT9.-sERaDEF.pGBT9—·sERa
transfectedintoY190 cells with for the invitro
yeast togetherplasmidpGAD424··TIF2establishinigsERa·-specificsystem
thesERa offour environmentalchemicals estrone
in wetested including
yeast.Then agonisticaffinity major estrogenic
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