鳜鱼两种谷胱甘肽S-转移酶基因cDNA的克隆与分析 Molecular Cloning and Sequence Analysis of Alpha-and Rho-Classes of Glutathione S-Transferases in Chinese perch(Sinipepca chuatsi).pdfVIP
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鳜鱼两种谷胱甘肽S-转移酶基因cDNA的克隆与分析 Molecular Cloning and Sequence Analysis of Alpha-and Rho-Classes of Glutathione S-Transferases in Chinese perch(Sinipepca chuatsi)
2009年第4卷 生 态 毒 理 学 报 V01.4,2009
第4期,537—543 AsianJournalofEcotoxicology No.4,537-543
鳜鱼两种谷胱甘肽S.转移酶基因cDNA的克隆与分析
程炜轩,梁旭方+,李观贵,王琳,杨宇晖
暨南大学生命科学技术学院,广州510632
chuatsi)是我国著名的肉食性鱼类,容易成为环境毒素的富集体.论文从分子水平上分析了鳜
摘要:鳜鱼(Siniperca
酸.系统分析结果显示:鳜鱼GSTA与GSTR在进化树上的位置与其分类所处的位置基本吻合.
关键词:谷胱甘肽s-转移酶;基因克隆;序列分析;鳜鱼
文章编号:1673—5897(2009)4—537—07 文献标识码:A
中图分类号:Q786
Molecularand of
CloningSequence Alpha-and
Analysis
in
ofGlutathioneS-TransferasesChinese chuatsi)
perch(Sinipelca
CHENG Yu—hui
Wei-xuan,LIANG Lin,YANG
Xu—fang+,LIGuan-gui,WANG
ofLifeScienceand 510632
College Technology,JinanUmversity,Guangzhou
Received3December2008 17March2009
accepted
an contaminatedtoxin.This
Abstract:As chuatsi)was
ac锄廿lomorphfish,Chinc∞perch(Sinoercaeasily by paper
thestructureandevolution of all
soluble S-ll阻nsferase(GST)inChinese
analyzed position ghtathione perch,whichphyod
roleindetoxification.Wethe andrho and fromtheliverof
GST(GSTAGSTR)eDNA
important got alpha sequences
Chinese RT—PCRandRACE.Resultsshowedthattotal ofGSTAandGSTRwere and
perchby length 1052bp935bp,
7
l 3’UTRand 5
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