荧光衍生物的合成毕业论文.doc

目 录 1.引言…………………………………………………………………………………2 1.1荧光分析技术…………………………………………………………………3 1.2 BODIY类染料研究历程…………………………………………………………3 1.3 BODIY类荧光染料的性能……………………………………………5 2.实验…………………………………………………………………………………6 2.1 实验仪器…………………………………………………………………………5 2.2 实验试剂…………………………………………………………………………6 2.3 实验原理…………………………………………………………………………6 2.4 实验步骤…………………………………………………………………………6 2.5 实验谱图…………………………………………………………………………7 3.讨论………………………………………………………………………………11 参考文献……………………………………………………………………………11 致 谢…………………………………………………………………………………12 BODIPY类荧光衍生物的合成 张萍，安徽师范大学化学与材料科学学院 摘要： 氮杂氟硼荧光染料（Aza-BODIPY）是一种新型荧光化合物，具有较高的的荧光量子产率和摩尔消光系数，并且表现出很好的光稳定性；可以用于设计荧光探针。作为其核心，BODIPY类荧光化合物合成具有重要意义，尤其是具有长波吸收的荧光化合物,由于对生物体伤害小,一直是人们关注的焦点。本论文主要设用4-甲氧基苯乙酮、对溴苯甲醛、硝基甲烷、乙二胺、醋酸铵、三氟化硼乙醚为原料，然后经过一系列反映得到Aza-BODIPY衍生物。 关键字： Aza-BODIPY；合成；荧光染料 Synthesis of BODIPY Derivatives Zhang ping, College of Chemistry and Materials Science Abstract: Aza-BODIPY fluorescent dyes,which are a new kind of fluorescent compounds, has high fluorescence quantity when zi chan rate and Moore extinction coefficient and that shows excellent light stability; and can be used to design fluorospectrophotometric probes。As its core, BODIPY class fluorescence compounds synthesis has an important meaning, and especially the fluorescence compounds with longwave absorption, because of small organisms, is always the damage the focus of attention. Aza-BODIPY fluorescent dyes,which are a new kind of fluorescent compounds, are widely used due to their outstanding advantages.This thesis mainly designs using 4-Methoxyacetophone， 2-Bromobenzal dehyde，nitromethane， diethylamine，ammonium acetate ，diisopropylethylamine and borontrifluoride diethyletherate to synthesis BODIPY derivatives Aza - a series. Key words: Aza-BODIPY；Synthesis; Fluorescent dyes. 引 言 1.1 荧光分析技术 目前应用最为广泛的一种有效生物检测方法就是荧光分析技术，这种技术是利用带有识别基团的荧光化合物分子作为检测试剂，在与靶标物结合的前后荧光的强度或者波长的变化来判定靶标的种类和浓度等等相关参数。荧光化合物能够发出光信号,而且对生物体无伤害作用,是完成这一任务的理想选择,所以荧光化合