星点设计-响应面分析法优选香薷提取工艺Δ-中国药房.PDFVIP

星点设计-响应面分析法优选香薷提取工艺Δ ＊ 刘颖新 ，刘利利，邱艳明，孔兴欣（湖南中医药高等专科学校药学系，湖南株洲 412012） 中图分类号 R914；R284 文献标志码 A 文章编号 1001-0408（2014）19-1757-04 DOI 10.6039/j.issn. 1001-0408.2014.19.09 摘 要 目的：优选香薷的提取工艺。方法：以乙醇体积分数、提取时间、溶媒比为自变量，香荆芥酚含量、麝香草酚含量为因变 量，通过对自变量各水平进行多元线性回归及二项式拟合，采用星点设计响应面分析法优选香薷提取工艺。结果：最佳提取工艺 为11倍量75％乙醇提取3次，每次70min ，该工艺条件下，香荆芥酚质量分数为0.303％、麝香草酚质量分数为0.722％。结论：星 点设计-响应面分析法优选的香薷提取工艺，方法简便，合理可行。 关键词 香薷；星点设计；响应面分析法；香荆芥酚；麝香草酚 Optimization of Extraction Technology for Mosla Herba by Central Composite Design-response Surface Meth- od LIU Ying-xin ，LIU Li-li ，QIU Yan-ming ，KONG Xing-xin （Dept. of Pharmacy ，Hunan Traditional Chinese Medical College ，Hunan Zhuzhou 412012，China ） ABSTRACT OBJECTIVE ：To optimize extraction process of Mosla Herba. METHODS ：The extraction process of Mosla Herba was optimized by central composite-response surface method. Taking the ethanol concentration ，extraction time and the amount of solvent as independent variables ，and the content of carvacrol and thymol as dependent variable ，each level of independent vari- ables were fitted by multiple linear regression and binomial formula ；The extraction process of Mosla Herba was optimized by re- sponse surface method. RESULTS ：Optimal extraction technology was as follows ：11-folds of 75％ ethanol ，extracting for 3 times ，70min each time. The concentration of carvacrol was 0.303％and that of binomial formula was 0.722％. CONCLUSIONS ： The extraction technology of Mosla Herba optimized by central composite-response surface method is simple ，reasonable. KEYWORDS Mosla Herba ；Central composite design ；Response surface method ；Carvacrol ；Thymol 续表8 脱干膏收率，多指标评价中药复方制剂精制工艺参数，在70％乙 Continued tab 8 醇洗脱液中，10个指标成分含量尽量高的前提下，干膏收率越