胰腺癌肝转移相关分泌蛋白质的筛选.docVIP

胰腺癌肝转移相关分泌蛋白质的筛选 【摘要】 目的：比较人胰腺癌不同肝转移潜能细胞株分泌蛋白质谱的差异，筛选鉴定与胰腺癌肝转移相关的分泌蛋白质。方法：应用双向电泳技术分离一对来自同一亲本、具有不同肝转移潜能的人胰腺癌细胞株L3.6 pl和Colo357的分泌蛋白质，图像分析软件筛选出两者差异表达的蛋白质点,利用基质辅助激光解析电离飞行时间质谱(MALDITOFMS)对部分差异蛋白质点进行鉴定。结果：建立了L3.6 pl和Colo357细胞分泌蛋白质的双向电泳图谱，识别出差异表达的蛋白质点20个，质谱鉴定出5个蛋白质点，其中组织蛋白酶D前体、表皮型脂肪酸结合蛋白在高肝转移潜能细胞株L3.6 pl中的表达水平高于低肝转移潜能细胞株Colo357，而视黄醛脱氢酶1、二磷酸核苷激酶B、膜连蛋白A1在L3.6 pl细胞株中的表达水平低于Colo357细胞株。结论：高、低不同肝转移潜能的胰腺癌细胞株分泌蛋白质的表达存在明显差异，两者间的差异蛋白可能与胰腺癌肝转移密切相关，胰腺癌肝侵袭转移的特性可能是诸多蛋白共同作用的结果。 【关键词】 胰腺癌; 肝转移; 分泌蛋白质; 双向电泳; 质谱 　　[Abstract] Objective: To compare differential secretory protein expression profiles of human pancreatic cell lines with different liver metastasis potentials for screening and identifing the secretory proteins associated with liver metastasis of pancreatic cancer. Methods: The secretory proteins of a pair of pancreatic cell lines L3.6 pl and Colo357 with different liver metastatic potentials and the same genetic background were separated by twodimensional gel electrophoresis(2DE)， respectively. Image master software was applied to analyze 2DE images，some of the differentially expressed protein spots between L3.6 pl and Colo357 were identified by matrixassisted laser desorption/ionizafion time of flight mass spectrometry(MALDITOFMS). Results: 2DE maps of secretory proteins from L3.6 pl and Colo357 were established.Twenty differentially expressed protein spots were identified by image master software， five of them were identified by MS. Cathepsin D precursor and epidermalfatty acidbinding protein were significantly higher in highly metastatic L3.6 pl than those in lowmetastatic Colo357， and other differential proteins including retinal dehydrogenase 1, nucleoside diphosphate kinase B and annexin A1 were significantly lower in highly metastatic L3.6 pl than that in lowmetastatic Colo357. Conclusion: The highliver metastatic pancreatic cancer cell line L3.6 pl display different secretory protein profiles compared to the lowliver metastatic pancreatic