血管内皮生长因子与细胞支架复合物修复大鼠股骨缺损.docVIP

血管内皮生长因子与细胞支架复合物修复大鼠股骨缺损 作者：徐成振, 马鹏, 徐晓峰, 李阳, 钱栋 【摘要】 目的： 探讨血管内皮生长因子(VEGF)与骨髓间充质干细胞（MSCs）、纳米晶胶原基骨(nHAC)复合物对大鼠股骨缺损的修复作用。方法： 将20只SD大鼠制成股骨缺损模型后分2组：对照组植入MSCs/nHAC复合物，实验组植入VEGF/MSCs/nHAC复合物。术后第2，4，8周行影像学和组织学观察;术后第8周行新生骨痂环境扫描电镜（ESEM）检查。结果： 术后第2，4，8周实验组与对照组放射学检查评价骨生成差异有统计学意义(Plt;0.05)。组织学观察发现实验组较对照组能更快更有效地促进大鼠股骨缺损处的骨痂生长。结论： VEGF/MSCs/nHAC支架较MSCs/nHAC支架对骨缺损的修复有更好的效果。 【关键词】 血管内皮生长因子； 骨髓间充质干细胞； 纳米晶胶原基骨； 骨缺损； 环境扫描电镜 ［Abstract］ Objective: To evaluate the effect of vascular endothelial growth factor（VEGF）combined with the compound of marrow mesenchymal stem cells(MSCs) and nanohydroxyapatite/collagen(nHAC) bone on the treatment of rat femoral bone defects.Methods： Animal models of femoral bone defect were established in 20 SpragueDawley rats.They were divided into 2 groups randomly: In control group，defect was filled with nHAC/MSCs scaffolds; in experiment group was filled with MSCs/VEGF/nHAC.Imaging and histological observation were made in the 2nd，4th and 8th weeks after operation.Environment scanning electron microscope(ESEM) observation was made in the 8th week after operation. Results： There were significant differences in bone regeneration between control group and experiment group in the 2nd，4th and 8th weeks after operation(Plt;0.05).Histomorphology observation showed that new osteotylus of experiment group was earlier and more than control group. Conclusion： The bone defect repair ability of tissueengineering bone which constructed with VEGF / MSCs/ nHAC was stronger than MSCs/ nHAC. ［Key words］ vascular endothelial growth factor; marrow mesenchymal stem cells; nanohydroxyapatite/collagen; bone defect; environment scanning electron microscope 骨缺损的修复仍然是当前临床骨科面临的难题之一。目前常用的手术治疗方式主要有自体骨移植、异体骨移植和人工骨移植等。骨组织工程学的出现，为这一难题提出了新思路，现阶段骨组织工程学的研究主要集中在种子细胞、支架材料和构建方式三个方面。骨髓间充质干细胞（marrow mesenchymal stem cells, MSCs）是最有应用前景的种子细胞, 纳米晶胶原基骨(nanohydroxyapatite/collagen， nHAC)也已经作为骨缺损代用人工合成生物支架材料应用于临床，研究表明其复合MSCs后具有体内成骨能力［1］。本实验选用MSCs为种子细胞与nHAC支架培养后复合血管内皮生长因子(vascular endoth