深圳海域6株破囊壶菌的生长特性及油脂成分分析.PDFVIP

微生物学通报 Jan. 20, 2015, 42(1): 17−23 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140415 研究报告 深圳海域 6 株破囊壶菌的生长特性及油脂成分分析 李晶晶 刘瑛 成家杨 Maurycy Daroch* ( 518055) 摘 要【目的】从深圳海域分离得到6 株破囊壶菌，对其基本形态特征、生活史和油脂含量 等进行研究，开发其应用潜力。【方法】使用松花粉垂钓法对破囊壶菌进行分离，通过 18S rRNA 基因测序的方法对破囊壶菌进行鉴定，用显微镜观察其基本形态特征，通过使用尼罗红(Nile Red)染色法对油脂含量进行定性检测，并用GC-MS 分析菌株的油脂含量和组成情况。【结果】 18S rRNA 基因鉴定其属于Aurantiochytrium sp. 、Schizochytrium sp.和Thraustochytrium sp.三个属。 破囊壶菌的脂肪酸主要成分为十六碳饱和脂肪酸和二十二碳六烯酸(DHA) ，其中Mn11 和Mn15 的饱和脂肪酸含量达到总脂肪酸含量的70%以上，Mn16 和Sw7 的DHA 产量分别达到 1.29 g/L 和1.26 g/L。【结论】Mn11 和Mn15 菌株适合用于生物柴油的生产，Mn16 和Sw7 是DHA 发酵 生产的潜力菌株。 关键词破囊壶菌，生活史，脂肪酸，生物柴油，DHA Growth features and fatty acid analysis of six thraustochytrid strains from Shenzhen coastal seawater * LI Jing-Jing LIU Ying CHENG Jia-Yang Maurycy Daroch (School of Environment and Energy, Peking University, Shenzhen, Guangdong 518055, China) Abstract: [Objective] Identified six thraustochytrid strains isolated from Shenzhen costal seawater and sediment. Morphological characteristics, life cycle and fatty acid profiles of these strains were studied. [Methods] Thraustochytrid strains were isolated using pine pollen as bait and identified based on 18S rRNA gene sequences. Morphological characteristics were observed using microscope. Nile red staining was used to qualitatively analyze oil content. While GC-MS were used to analyze specific fatty acid profile. [Results] Analysis in 18S rRNA gene rev