改良分子信标-实时PCR快速检测产单核李斯特菌.PDFVIP

644 2006 6 16 6 C inese Journal ofH ea lt Laboratory Tec nology, Jun 2006; V ol 16 No 6 改良分子信标- 实时 PCR快速检测产单核李斯特菌 1 1 1 2 2 1 1 1 王 冰, 扈庆华 , 石晓路 , 李庆阁, 郑琳琳 , 林一曼, 贺连华 , 张顺祥 ( 11 , 518020; 21 , 361005) [ ] : - PCR( LMO), LMO : GenBank LMO lyA , , - PCR, LMO: - PCR DNA 110 fg, 99 cfu/m l 4 cfu/PCR , 28 LMO, 4 d 1 d 228 LMO, 8 LMO PCR, 6 LMO: - PCR, , LMO, LMO [ ] 产单核李斯特菌; 改良分子信 ; 实时 PCR; 检测 + [ ] R378199 4 [] A [ ] 1004- 8685( 2006) 06- 0644- 03 Rapid simultaneous detection ofL isteria m onocy tog enes usingmodifiedmolecular beacons and real- timePCR 1 1 1 2 2 1 1 1 W ang B in , H u Q ing-hua , Sh iX iao-lu , L i Q ing-g e , Zheng L in-lin , L in Y i-m an , H e L ian-hua , Zhang Shun-x iang ( 11S enz en Center forD iseaseControl and Prevention, S enz en 518020, C ina; 21Sc ool ofL ife Sciences, X iam en University, X iam en 361005, C ina) [Abstract] Objective:Rapid detection ofL is teria monocy tog enes ( LMO) using m od ified m olecu lar beacons and real- tmi e PCR w as developed1T e estab lis ed m et odw as applied to an investigation of t e infectious status ofL isteria m onocy tog enes in food and LMO food poisoning1Methods:One set of prmi ersw as designed based on t e core sequence of lyA gene pub lis ed on GenB ank to detect LMO1T e correspond ing mod ified molecular beacons labeled w it FAM were designed1T em odified molecular beacons and real- tmi e PCR assay w ere app lied to detect t e LMO in food1Results:For t