Resveratrol enhances GLUT 4 translocation to the caveolar lipid raft fractions through AMPK Akt eNOS signalling pathway in diabetic myocardium英文文献.pdfVIP

J. Cell. Mol. Med. Vol 12, No 6A, 2008 pp. 2350-2361 Resveratrol enhances GLUT-4 translocation to the caveolar lipid raft fractions through AMPK/Akt/eNOS signalling pathway in diabetic myocardium S. Varma Penumathsa, a, c M. Thirunavukkarasu, a L. Zhan, a G. Maulik, b c d a V. P. Menon, D. Bagchi, N. Maulik a Molecular Cardiology and Angiogenesis Laboratory, Department of Surgery, University of Connecticut Health Center, Farmington, CT, USA b Department of Thoracic Surgery, Harvard Medical School, Boston, MA, USA c Department of Biochemistry and Biotechnology, Annamalai University, TN, India d Interhealth Research Center, Benicia, CA, USA Received: July 16, 2007; Accepted: January 23, 2008 Abstract Homeostasis of blood glucose by insulin involves stimulation of glucose uptake by translocation of glucose transporter Glut-4 from intracellular pool to the caveolar membrane system. In this study we examined resveratrol (RSV)-mediated Glut-4 translocation in the streptozotocin (STZ)-induced diabetic myocardium. The rats were randomized into three groups: Control (Con), Diabetes Mellitus (DM) (STZ 65 mg/kg b.w., i.p.) DM + RSV (2.5 mg/kg b.wt. for 2 weeks orally) (RSV). Isolated rat hearts were used as per the experimental model. RSV induced glucose uptake was observed in vitro with H9c2 cardiac myoblast cel