Differences in gene expression between natural and artificially induced leaf senescence英文文献.pdfVIP

Planta (1993)189:74-79 P I ~ I ] _ ~ 9 Springer-Verlag1993 Differences in gene expression between natural and artificially induced leaf senescence Walter Becker i . and Klaus Apel 2 ** t BotanischesInstitut der Universit/itKiel,Olshausenstrasse40-60, W-2300Kiel,FRG z Institut fiir Pflanzenwissenschaften,ETH Ziirich,Universitfitsstrasse2, CH-8092Ziirich, Switzerland Received25 May; accepted30 June 1992 Abstract. Gene expression during artificially induced se- Introduction nescence of barley ( H o r d e u m vulgate L.) leaves was ex- amined by in-vitro translation and mRNA hybridization Leaf senescence is the sequence of events leading to cellu- with several copy-DNA (cDNA) clones for newly in- lar disassembly and the mobilization of released materi- duced transcripts. When detached barley leaves were in- als that eventually results in the death of the organ cubated in darkness, senescence symptoms as indicated (Stoddart and Thomas 1982). As a consequence, nearly by chlorophyll loss were rapidly induced. By in-vitro all of the cellular nitrogen and other minerals are remo- translation, concomitant changes in translatable mRNA bilized and transported to sinks of the plant, such as levels were shown to occur with some translation prod- growing leaves or developing seeds. The apparent physi- ucts decreasing and others increasing in abundance. For ological advantage of this recycling process leads one closer analysis, cDNA clones for newly induced tran- to vi