A Mesenchymal to Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts英文文献.pdfVIP

Cell Stem Cell Article A Mesenchymal-to-Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts Ronghui Li,1,2 Jialiang Liang,1,2 Su Ni,1 Ting Zhou,1 Xiaobing Qing,1 Huapeng Li,1 Wenzhi He,1 Jiekai Chen,1 Feng Li,1 Qiang Zhuang,1 Baoming Qin,1 Jianyong Xu,1 Wen Li,1 Jiayin Yang,1 Yi Gan,1 Dajiang Qin,1 Shipeng Feng,1 Hong Song,1 Dongshan Yang,1 Biliang Zhang,1 Lingwen Zeng,1 Liangxue Lai,1 Miguel Angel Esteban,1,* and Duanqing Pei1,* 1Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China 2These authors contributed equally to this work *Correspondence: esteban@gibh.org (M.A.E.), pei_duanqing@gibh.ac.cn (D.P.) DOI 10.1016/j.stem.2010.04.014 SUMMARY the appearance of iPSCs takes at least 12–24 days and is very inefficient, nuclear reprogramming is considered a stochastic Epithelial-to-mesenchymal transition (EMT) is a process in which successive barriers must be overcome to reach developmental process important for cell fate deter- a state toward pluripotency (Hanna et al., 2009b; Yamanaka, mination. Fibroblasts, a product of EMT, can be reset 2009), but the nature of these barriers is poorly understood. into induced pluripotent stem cells (iPSCs) via exog- Remarkably, a series of compounds including TGF-b (transform- enous transcription factors but the underlying mech- ing growth factor b) inhibitors have been identified that accel- erate or improve the reprogrammin