Functionally distinct subsets of human NK cells and monocyte DC like cells identified by coexpression of CD56, CD7, and CD4英文文献.pdfVIP

IMMUNOBIOLOGY Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4 Jeffrey M. Milush,1 Brian R. Long,1 Jennifer E. Snyder-Cappione,1 Amedeo J. Cappione III,2 Vanessa A. York,1 Lishomwa C. Ndhlovu,1 Lewis L. Lanier,3 Jakob Michae¨lsson,4 and Douglas F. Nixon1 1Division of Experimental Medicine, Department of Medicine, University of California, San Francisco; 2Guava Technologies Inc, Hayward, CA; 3Department of Microbiology and Immunology and the Cancer Research Institute, University of California, San Francisco; and 4Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden The lack of natural killer (NK) cell– CD7CD56 NK cells, CD7negCD56 cells or K562 target cell stimulation. Further- specific markers, as well as the overlap lack expression of NK cell–associated more, using CD7 to separate CD56 NK among several common surface anti- markers, but share commonalities in cells and CD56 myeloid cells, we dem- gens and functional properties, has ob- their expression of various monocyte/ onstrate that unlike resting CD7CD56 scured the delineation between NK cells DC-associated markers. Using CD7, we NK cells, the CD7negCD56 myeloid cells and dendritic cells. Here, novel subsets observed approximately 60% of stimulate a potent allogeneic response. of peripheral blood CD3/14/19neg NK cells CD4CD56 cells were CD7neg cells, indi- Our data indicate that CD7 and CD56 and monocyte/dendritic cell (DC)–like cating the actual frequency of activated coexpression discriminates NK cells cells were identified on the basis of CD7 CD4 NK cell