N arachidonyl glycine modulates synaptic transmission in superficial dorsal horn英文文献.pdfVIP

Page 1 N-arachidonyl-glycine modulates synaptic transmission in superficial dorsal horn* Hyo-Jin Jeong*,1 2 1 , Robert J Vandenberg Christopher W Vaughan 1Pain Management Research Institute, Kolling Institute of Medical Research, Northern Clinical School, University of Sydney at Royal North Shore Hospital, NSW, Australia; 2Department of Pharmacology, Bosch Institute, University of Sydney, NSW, Australia. Running title: NAGly enhances glycine transmission in dorsal horn *Correspondence should be addressed to: Dr Hyo-Jin Jeong Pain Management Research Institute Kolling Institute of Medical Research, Northern Clinical School University of Sydney at Royal North Shore Hospital, St Leonards, NSW 2065, Australia E-mail: jeong@.au Phone: +61-2-9926 4965. Fax: +61-2-9906 4079. URL: .au This is an Accepted Article that has been peer-reviewed and approved for publication in the British Journal of Pharmacology, but has yet to undergo copy-editing and proof correction. Please cite this article as an “Accepted Article”; doi: 10.1111/j.1476-5381.2010.00935.x Page 2 SUMMARY Background and purpose. The arachidonyl-amino acid N-arachidonyl glycine (NAGly) is an endogenous lipid, generated within the spinal cord and producing spinally mediated analgesia via non-cannabinoid mechanisms. In this study we examined the actions of NAGly on neurons within the superficial dorsal horn, a key site for the actions of many analgesic agents. Experimental approach. Whole cell patch clamp recordings were made from lamina II neurons in rat spinal cord slices to examine the effect of NAGly on glycinergic and NMDA-mediated synaptic transmission. Key results. NAGly prolonged the decay of glycine, but