Rapid determination of serological cytokine biomarkers for hepatitis B virus related hepatocellular carcinoma using antibody microarrays英文文献.pdfVIP

ActaBiochim BiophysSin2011，43：45—51 ⑥『TheAuthor2010PublishedbyABBSEditorialOfficeinassociationwithOxfordUniversityPressonbehalfofthe InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences，ChineseAcademyofSciencesDOl：101093／abbs／gmq111 A AdvanceAccessPublication6December2010 OriginalArticle RapiddeterminationofserologicalcytokinebiomarkersforhepatitisB virus-related hepatocellularcarcinomausingantibodymicroarrays TaotaoLiu ，RuyiXue ，LingDong，HapWu，DanyingZhang，andXizhongShen DepartmentofGastroenterologyandHepatology，ZhongshanHospital，FudanUniversity，Shanghai200032，China 十Theseauthorscontributedequallytothiswork ． Correspondenceaddress．Tel：+86—212070；Fax：+86—21E—mail：shen．xizhong@zs—hospita1．sh．cn HepatoceIlularcarcinoma (HCC)isoneofthemostfre— Introduction quenttumorsworldwide with an increasing incidence． The exploration ofbiomarkersforHCC is one ofthe HepatocellularcarcinomarHcc)iSoneofthemostfre． main aimsofr improving the effi cacy ofdiagnosis and quent tumors worldwide with an increasing incidence． treatment．The microarray technology providesa high- Diagnosis．treatment．andprevention OfHCC imposeone throughput platform ofr parallel exploration of ofthemostwidely concernedpublichealthproblemsin biomarkersofr clinics．In this study，we used antibody manycountries 1『，21．Sofar，themostwidelyusedserum m icroarraysto screen thenovelcytokiHebiomarkersof biomarkerforHCC screeningisalpha．fetoprotein (AFP) hepatitisBvirus(HBV)-relatedHCC．Cytokine-secreting thatisincreasedr20ng／m1)in 30-60％ patientswith patternsin seraweredeterm inedfrom 109casesincluding HCC．Asaconsiderablenumberofpatientswith chronic 43 HBV．related HCC patients．33 chronic hepatitisB liver disease display AFP levels in the range of 20- patients．and33normalcontro