Enantioselective Oxidative Damage of Chiral Pesticide Dichlorprop to Maize英文文献.pdfVIP

ARTICLE pubs.acs.org/JAFC Enantioselective Oxidative Damage of Chiral Pesticide Dichlorprop to Maize Tong Wu, Xiuying Li, Honglin Huang, and Shuzhen Zhang* State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People’s Republic of China ABSTRACT: To investigate the enantioselective oxidative damage of the pesticide dichlorprop (DCPP) to maize, young seedlings were exposed to solutions of DCPP enantiomers and racemate at different concentrations. Early root development was more influenced by (R)-DCPP than racemic (rac)- and (S)-DCPP. Inhibition rates of seed germination, seedling biomass, and root and shoot elongation were all in the order of (R)-DCPP (rac)-DCPP (S)-DCPP treatments. The antioxidant enzyme activities of superoxide dismutase (SOD) and peroxidase (POD) were significantly upregulated by exposure to lower concentrations of (R)- DCPP than (rac)- and (S)-DCPP. Direct determination of the formation of hydroxyl radical (•OH) with electron paramagnetic resonance (EPR) spectroscopy indicated that the •OH level in maize roots followed the order of (R)-DCPP (rac)-DCPP (S)- DCPP treatments. All of these results provide solicited evidence of the significant enantioselective phytotoxicity of DCPP to maize with a higher toxicity of (R)-DCPP than (S)- and (rac)-DCPP. KEYWORDS: Oxidative damage, enantioselective phytotoxicity, dichlorprop (DCPP), hydroxyl radical ’INTRODUCTION mechanisms to deal with the damaging effects of ROS in cellular Many modern pesticides are chiral com