原创力文档A Novel Biotinylated Heterobifunctional Cross linking Reagent Bearing an Aromatic Diazirine英文文献.pdfVIP
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Pergamon Bioorganic Medicinal Chemistry, Vol. 2, No. 12, pp. 1367-1373, 1994
Copyright 8 1995Elsevler Science Limited
Printed in Great Britain. All rights reserved
0968-0896l94$7.00 + .OO
0968-0896(94)00129-4
A Novel Biotinylated Heterobifunctional Cross-linking Reagent
Bearing an Aromatic Diazirine
Yasumaru Hatanaka,l* Makoto Hashimoto2 and Yuichi Kanaoka3
‘Research Institute for Wakan- Yaku, Toyama Medical and Pharmaceutical University, Sugitani 2630, Toyama, 930-01
Japan 2Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060 Japan 3Toyama Women% College, 444
Gankaiji, Toyama, 930-01 Japan
Abstract-The synthesis of a p-[(3-triIluoromethyl)diazirine-3-yllbenzoic acid derivative is described as a new carbene generating
heterobifunctional cross-linking reagent. The cross-linker carries a biotin moiety in order to make use of avidin-biotin technology
for specific manipulation of cross-linked components. To evaluate the ability of this reagent, the inter-subunit cross-linking of
egg-white avidin tetramer was investigated. As a typical application of avidir-biotin technology for cross-linki
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